EPI001
Histone Acetyltransferase (HAT) Activity Assay Kit
100 assays in 96 well plates
Synonym(s):
HAT Activity, Histone Acetyltransferase Activity
usage
100 assays in 96 well plates
shipped in
wet ice
storage temp.
−20°C
General description
Histone acetyltransferases (HATs) have critical roles in cellular functions and are involved in the regulation of gene transcription, differentiation, and proliferation. The HAT Activity Colorimetric Assay Kit offers a convenient, non-radioactive system for a rapid and sensitive detection of HAT activity in mammalian samples. The kit includes an active Nuclear Extract (NE) to be used as a positive control, HAT cofactor, acetyl-CoA, and all downstream cofactors and substrates. Acetylation of a peptide substrate by active HAT releases the free form of CoA, which then serves as an essential coenzyme for producing NADH. NADH can easily be detected spectrophotometrically upon reacting with a soluble tetrazolium dye. The detection can be continuous, and therefore, is suitable for kinetic studies. The kit provides all reagents and a simple, straightforward protocol for a complete assay.
Features and Benefits
- Sensitive and reliable assay
- Utilizes colorimetric methods
- Sample type: cell and tissue lysates
- Species reactivity: mammalian
- Suitable for individual tests or high throughput assays and kinetic studies
- Easy and simple procedure: combine nuclear extract or protein sample with reagents, incubate, and read absorbance
- Convenient 96-well microplate format
- Continuously measures HAT activity; suitable for kinetic studies
- No HDAC interference; crude nuclear extract can be used directly
Storage Class
12 - Non Combustible Liquids
Regulatory Information
监管及禁止进口产品
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Hiroshi Watanabe et al.
Nihon rinsho. Japanese journal of clinical medicine, 71(12), 2091-2096 (2014-01-21)
Aging alters a broad spectrum of physiological, endocrine, and behavioral rhythms, and the close relationship between age-associated disease and disrupted circadian rhythms has been shown. Circadian rhythms are regulated by a set of clock genes and mutations in clock genes
Carolina Alquezar et al.
Science advances, 7(45), eabg3897-eabg3897 (2021-11-06)
Age-associated neurodegenerative disorders demonstrating tau-laden intracellular inclusions are known as tauopathies. We previously linked a loss-of-function mutation in the TSC1 gene to tau accumulation and frontotemporal lobar degeneration. Now, we have identified genetic variants in TSC1 that decrease TSC1/hamartin levels
Michiko Konno
Nihon rinsho. Japanese journal of clinical medicine, 71(12), 2171-2178 (2014-01-21)
Mood disorders show a common feature of distorted cycling of biological systems, manifesting, for example, as diurnal mood variation in depression, phasic time course in bipolar disorder, and seasonal mood swing in seasonal affective disorder. Accordingly, circadian dysfunction has been
Sydney P Thomas et al.
eLife, 10 (2021-10-23)
Short-chain fatty acids (SCFAs) acetate, propionate, and butyrate are produced in large quantities by the gut microbiome and contribute to a wide array of physiological processes. While the underlying mechanisms are largely unknown, many effects of SCFAs have been traced
Igor Efimov et al.
FEBS letters, 588(5), 701-704 (2014-01-21)
We describe a simple method for the determination of heme protein reduction potentials. We use the method to determine the reduction potentials for the PAS-A domains of the regulatory heme proteins human NPAS2 (Em=-115 mV ± 2 mV, pH 7.0)
Related Content
Instructions
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service