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Merck
CN

F137

Sigma-Aldrich

Anti-c-Fos antibody produced in rabbit

IgG fraction of antiserum, lyophilized powder

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About This Item

MDL number:
UNSPSC Code:
12352203
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biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

mol wt

antigen 50-60 kDa

species reactivity

mouse

technique(s)

western blot (chemiluminescent): 10 μg/mL using mouse NIH3T3 cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... FOS(2353)
mouse ... Fos(14281)

Immunogen

synthetic peptide derived from amino acids 4-17 from the N-terminal region of human and mouse c-Fos.

Application

Anti-c-Fos antibody produced in rabbit is suitable for the following applications:
  • Western blotting (chemiluminescent) at a concentration of 10μg/mL using mouse NIH3T3 cells
  • Immunohistochemistry
  • Tissue Immunofluorescence

Biochem/physiol Actions

The antibody does not react with c-Fos in HeLa cells.
c-Fos is a proto-oncogene, which is the human homolog of the retroviral oncogene v-fos. It is one of the essential transcription factors that helps in initiation of adipocyte differentiation. FOS protein expression in human glioma is found to be strong and probably plays a critical role in the progression of gliomas. c-Fos may be involved in regulating lipid synthesis. c-Fos is associated with prognosis of ovarian cancer (OvCa) by changing the adhesion of OvCa cells to peritoneal surfaces.

Physical form

Lyophilized from phosphate buffered saline containing 0.05% sodium azide.

Preparation Note

Prepared using protein A.
Reconstitute with 100 μl deionized water.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

13 - Non Combustible Solids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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D S Su et al.
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