Monoclonal Anti-hFBXW7γ antibody produced in mouse

FBXW7 is a F-box protein that facilitates as a substrate recognition component of the ubiquitin ligase complex SCF (Skp-Cullin-F-box). FBXW7 exist in three isoforms α, β and γ. It is composed of a conserved F-box domain at their N terminus, which interacts with Skp1. C terminus consist of one or several highly variable protein-protein interaction domains such as Leu-rich repeat (LRR), kelch repeat, tetratricopeptide repeat (TPR), and WD40 repeat.

synthetic peptide corresponding to amino-acids 13-27 of human hFBXW7γ isoform.

Monoclonal Anti-hFBXW7γ antibody is suitable for:-

• Immunocytochemistry at a concentration of 10-20μg/mL using human A431 cells.
• Western blot at a concentration of 5-10μg/mL using human A431 cell extracts.
• Indirect ELISA.

FBXW7 protein is highly involved in the ubiquitination of cyclin E , Notch , c-Jun and c-Myc. It acts as a key component of phosphoepitope-specific substrate recognition complex of SCF ubiquitin ligases to facilitate the recognition of phosphorylated targets such as cyclin E, myc, c-Jun, and Notch. The SV40 large T antigen binds FBXW7 without detectible effects on its stability to acts as an inhibitor of FBXW7 activity.It has been reported that FBXW7 controls c-Myc′s growth-promoting activity followed by cell size, which is an important event of Fbw7 loss in cancers.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.