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Merck
CN

F2561

Sigma-Aldrich

Fetal Bovine Serum

sterile-filtered, suitable for cell culture

Synonym(s):

FBS, FCS, sera, serum

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About This Item

MDL number:
MFCD00132239
UNSPSC Code:
12352200

Key Documents

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biological source

bovine fetus

Quality Level

500

sterility

sterile-filtered

product line

FBS Classic

composition

Hemoglobin, ≤25 mg/dL

origin

Brazil origin

availability

available only in Brazil

technique(s)

cell culture | mammalian: suitable

impurities

≤5 EU/mL Endotoxin

shipped in

dry ice

storage temp.

−20°C

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General description

Fetal Bovine Serum (FBS) is a complex mixture with low and high molecular weight biomolecules exhibiting balanced growth-promoting and growth-inhibiting functions. FBS contains molecules, such as growth factors, proteins, vitamins, trace elements, hormones etc. These molecules are important for the growth and maintenance of cells. It is a common supplement for cell culture media.

Application

FBS derived from clotted blood is the most widely used undefined supplement in eucaryotic, especially mammalian, cell culture. It is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM); Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM); M199 and Roswell Park Memorial Institute medium, RPMI-1640.
Fetal bovine serum (FBS) is suitable for broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Legal Information

Not available in USA or Canada. Please check with your local supplier regarding availability and import restrictions in your country.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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FT-IR characterization of a theranostic nanoprobe for photodynamic therapy and epidermal growth factor receptor targets.
Castilho ML et al.
Sensors and Actuators B, Chemical, 240, 903-908 (2017)
Optimization of chemically defined cell culture media--replacing fetal bovine serum in mammalian in vitro methods.
van der Valk J et al.
Toxicology in vitro, 24, 1053-1053 (2010)
Alternatives to the use of fetal bovine serum: serum-free cell culture.
Gstraunthaler G
ALTEX, 20, 275-275 (2003)
Li Zhou et al.
Genome biology, 9(7), R119-R119 (2008-07-31)
CD8+ NKT-like cells are naturally occurring but rare T cells that express both T cell and natural killer cell markers. These cells may play key roles in establishing tolerance to self-antigens; however, their mechanism of action and molecular profiles are
Ruth M Risueño et al.
PloS one, 3(3), e1747-e1747 (2008-03-06)
How the T cell antigen receptor (TCR) discriminates between molecularly related peptide/Major Histocompatibility Complex (pMHC) ligands and converts this information into different possible signaling outcomes is still not understood. One current model proposes that strong pMHC ligands, but not weak
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