F4428
Fas Ligand human
>95%, recombinant, expressed in HEK 293 cells
Synonym(s):
Apo-1L, CD178, CD95L, FasL, TNFSF 6
recombinant
expressed in HEK 293 cells
Assay
>95%
usage
sufficient for 200-400 mL (medium containing Jurkat cells)
mol wt
26-32 kDa by SDS-PAGE (triplet due to different degrees of glycosylation)
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... FASLG(356)
Biochem/physiol Actions
Fas ligand, a protein belonging to the tumor necrosis factor (TNF) family of cytokines, induces apoptosis in cells expressing the cell membrane receptor Fas (CD95/Apo-1).
Fas ligand, a protein belonging to the tumor necrosis factor (TNF) family of cytokines, induces apoptosis in cells expressing the cell membrane receptor Fas (CD95/Apo-1). FasL is expressed mainly in lymphoid tissue such as spleen and thymus and in a few other non lympoid tissues including the eye, testis and thyroid.
FasL is involved in immune down regulation and in the process of negative selection of T cells in the peripheral lymphoid organs. It also plays an important role in conferring immuno-privilege to the non-lymphoid tissues, such as eye and testis, in which it is expressed. It is suggested that constitutive FasL expression in a variety of tumors contributes to their immune evasion.
It has been shown that FasL is also involved in mediating inflammatory processes and can have pro-proliferative effects. The dual activity of FasL can be explained by diversion of the FasL signal to apoptosis of proliferation/differentiation, down stream of its receptor.
FasL is involved in immune down regulation and in the process of negative selection of T cells in the peripheral lymphoid organs. It also plays an important role in conferring immuno-privilege to the non-lymphoid tissues, such as eye and testis, in which it is expressed. It is suggested that constitutive FasL expression in a variety of tumors contributes to their immune evasion.
It has been shown that FasL is also involved in mediating inflammatory processes and can have pro-proliferative effects. The dual activity of FasL can be explained by diversion of the FasL signal to apoptosis of proliferation/differentiation, down stream of its receptor.
Analysis Note
Activity: 0.5-5 ng/ml FasL induces apoptosis in Jurkat cells in the presence of 0.25-1 μg/ml ANTI-FLAG M2 monoclonal antibody as measured by caspase 3 activity.
This FLAG tagged FasL requires the presence of FLAG® antibody for its aggregation and activation. The FLAG antibody is included in the set.
This FLAG tagged FasL requires the presence of FLAG® antibody for its aggregation and activation. The FLAG antibody is included in the set.
Legal Information
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Kit Components Only
Product No.
Description
- Fas Ligand, human, expressed in HEK-293 cells (N-terminal FLAG-tagged) 10 μg
Kit Components Also Available Separately
Product No.
Description
SDS
- F3165Monoclonal ANTI-FLAG® M2, clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide) .2 mgSDS
Regulatory Information
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