F5027
Monoclonal Anti-IgD−FITC antibody produced in mouse
clone HJ9, purified immunoglobulin, buffered aqueous solution
Synonym(s):
Monoclonal Anti-Human IgD
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About This Item
UNSPSC Code:
12352203
Conjugate:
FITC conjugate
Clone:
HJ9, monoclonal
Application:
FACS
Citations:
2
biological source
mouse
conjugate
FITC conjugate
antibody form
purified immunoglobulin
antibody product type
secondary antibodies
clone
HJ9, monoclonal
form
buffered aqueous solution
species reactivity
human
technique(s)
flow cytometry: 10 μL using 1 × 106 cells
isotype
IgG1
UniProt accession no.
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... IGHD(3495)
Immunogen
human IgD
Application
Monoclonal Anti-IgD-FITC antibody is suitable for flow cytometry at a concentration of 10μL using 1×106 cells.
Biochem/physiol Actions
IgD is the major antigen receptor isotype coexpressed with IgM on the surface of human peripheral B cells. It is associated with early antibody response. It occupies approximately 0.25% of total immunoglobulins. In serum, it has shown that the catabolism of IgD is decreased at high concentrations. IgD molecule comprises of a long “hinge” region (between Fab and Fc) including three constant region domains. This makes the molecule very susceptible to proteolytic degradation with production of Fab and Fc fragments. It also helps to maintain the molecule flexibility that enhance antigen binding capacity. IgD is a potent inducer of tumor necrosis factor (TNF), Interlukin 1β(IL1β), and Interlukin IL1RN (IL1RN). It also accelerates the release of IL6, IL10 from peripheral blood mononuclear cells. Its increased level in serum causes hyper-IgD and periodic fever syndrome (HIDS).
Recognizes the delta chain of human IgD and is not reactive with heavy chains from human IgG1, IgG2, IgG3, IgG4, IgM, IgA, or kappa and lambda light chains.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
Preparation Note
Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.
Other Notes
IgD is expressed on peripheral B cells and is used as a pan-B cell marker.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Immunoglobulin D: properties, measurement, and clinical relevance.
A O Vladutiu
Clinical and diagnostic laboratory immunology, 7(2), 131-140 (2000-03-07)
J P Drenth et al.
Immunology, 88(3), 355-362 (1996-07-01)
Immunoglobulin D (IgD) is normally present in only low concentrations in serum. In the hyper-IgD and periodic fever syndrome (HIDS), however, serum levels exceed 140 mg/l. This syndrome is further characterized by recurrent inflammatory febrile attacks together with an acute
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