F8674
Fas/Fc Chimera human
≥97% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture
Synonym(s):
Apo-1, CD95
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About This Item
MDL number:
UNSPSC Code:
12352200
recombinant
expressed in NSO cells
Assay
≥97% (SDS-PAGE)
form
lyophilized powder
potency
0.01-0.04 μg per mL ED50 (in the presence of 2 ng/ml recombinant human Fas ligand for cytotoxicty assay of Jurkat cells)
mol wt
45 kDa
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... FAS(355)
General description
Fas is a key component of apoptosis, and is a cell surface receptor. This gene is localized to human chromosome 10q24.1, and is composed of nine exons and eight introns.
Biochem/physiol Actions
Fas and its ligand play an essential role in apoptosis, by activating the cell death signaling pathway. Mutations in this gene are associated with evasion of apoptosis by cells, leading to tumorigenesis, and prevention of clearance of these cells by the immune system. A polymorphism in this gene results in the production of soluble Fas (sFas), which is implicated in the chronic autoimmune disorder called primary Sjögren′s syndrome (pSS).
Fas is a surface (trans-membrane) receptor that is a member of the TNF receptor/nerve growth factor receptor family. Fas receptor mediates apoptosis in a wide variety of cell types.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin
Analysis Note
Biological activity was measured by its ability to inhibit Fas-ligand induced apoptosis of Jurkat cells.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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J Cheng et al.
Science (New York, N.Y.), 263(5154), 1759-1762 (1994-03-25)
Fas is an apoptosis-signaling receptor molecule on the surface of a number of cell types. Molecular cloning and nucleotide sequence analysis revealed a human Fas messenger RNA variant capable of encoding a soluble Fas molecule lacking the transmembrane domain because
A Oehm et al.
The Journal of biological chemistry, 267(15), 10709-10715 (1992-05-25)
The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 was previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. Cross-linking of the
B A Treviño-Talavera et al.
Genetics and molecular research : GMR, 13(3), 4831-4838 (2014-07-26)
Primary Sjögren's syndrome (pSS) is a chronic systemic autoimmune disease characterized by lymphocytic infiltration of exocrine glands. Soluble Fas receptor (sFas) has been suggested as a Fas-mediated apoptosis blocker that could impair clonal deletion in infiltrated autoreactive cells. The FAS
N Itoh et al.
Cell, 66(2), 233-243 (1991-07-26)
Mouse anti-Fas monoclonal antibody has a cytolytic activity on human cells that express the antigen. Complementary DNAs encoding the cell surface antigen Fas were isolated from a cDNA library of human T cell lymphoma KT-3 cells. The nucleotide sequence of
Cheng Jin et al.
Medical science monitor : international medical journal of experimental and clinical research, 21, 630-637 (2015-02-28)
The CD95 gene plays a key role in regulating cell growth and tumor genesis. To date, several publications have focused on the CD95 rs1800682A/G site polymorphism and various types of tumors in Asians; however, this association is still controversial and
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