F9762
Anti-Human IgM (μ-chain specific)−FITC antibody produced in goat
affinity isolated antibody, buffered aqueous solution
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About This Item
MDL number:
UNSPSC Code:
12352203
biological source
goat
conjugate
FITC conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
storage condition
protect from light
technique(s)
immunofluorescence: suitable (1:32 - 1:64 using Hep2 cells)
storage temp.
−20°C
target post-translational modification
unmodified
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General description
IgM antibodies are present as pentamers in the serum and are produced in response to antigens
Anti-Human IgM (μ-chain specific)-FITC antibody specifically binds to mu-chain of human IgM. After immunospecific purification, anti-human IgM is conjugated to fluorescein isothiocyanate (FITC), in an alkaline reaction. The antibody preparation is specific for human IgM when tested against purified human IgA, IgG, IgM, Bence Jones κ and λ myeloma proteins. The product is also determined to have no cross reactivity with mouse or rat serum proteins.
Anti-Human IgM (μ-chain specific)-FITC antibody specifically binds to mu-chain of human IgM. After immunospecific purification, anti-human IgM is conjugated to fluorescein isothiocyanate (FITC), in an alkaline reaction. The antibody preparation is specific for human IgM when tested against purified human IgA, IgG, IgM, Bence Jones κ and λ myeloma proteins. The product is also determined to have no cross reactivity with mouse or rat serum proteins.
Immunogen
Purified human IgM
Application
Anti-Human IgM (μ-chain specific)-FITC antibody may be used for immunofluorescence at a working antibody dilution of 1:64 using human peripheral blood lymphocytes. A dilution of 1:32-1:64 may be used for anti-nuclear antibody (A.N.A.) assay on acetone-fixed human Hep2 cells. A dilution of 1:32 may be used on acetone-fixed mouse liver cells for anti-nuclear antibody assay. It may also be used for agar diffusion assay.
Biochem/physiol Actions
No cross-reaction with mouse and rat IgG.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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E J Wiersma et al.
Journal of immunology (Baltimore, Md. : 1950), 160(12), 5979-5989 (1998-06-24)
Previous studies have discerned two forms of polymeric mouse IgM: moderately cytolytic (complement-activating) pentamer, which contains J chain, and highly cytolytic hexamer, which lacks J chain. To investigate the relationships among polymeric structure, J chain content, and cytolytic activity, we
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