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Merck
CN

G0170

Sigma-Aldrich

Anti-GABAA Receptor (α4 subunit), N-Terminus antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Anti-γ-Aminobutyric Acid Type A Receptor (α4 subunit)

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About This Item

MDL number:
MFCD07370319
UNSPSC Code:
12352203
NACRES:
NA.41

Key Documents

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biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

usage

sufficient for 10 blots

mol wt

antigen (active form) ~64 kDa

species reactivity

rat

technique(s)

immunoprecipitation (IP): suitable
western blot: 1:1,000-1:2,000 using rat brain lysate

UniProt accession no.

P28471

shipped in

wet ice

storage temp.

−20°C

Gene Information

rat ... Gabra4(140675)

General description

Gamma-aminobutyric acid (GABA) receptor is the multifunctional, pentameric membrane proteins that operate GABA-gated chloride channels. It acts as major inhibitory neurotransmitter in the adult mammalian brain. It is a member of the neurotransmitter ligand-gated ion channels which plays various functions in the central nervous system, the peripheral nervous system, and in some non-neuronal tissues. At least four types of subunits (α, β, γ and δ) of GABA have been identified. Generally it contains one type of α and β subunit, and a single γ polypeptide in a ratio of 2:2:1.

Immunogen

synthetic peptide from the N-terminus of the rat GABAA receptor α4 subunit.

Application

Anti-GABAA Receptor (α4 subunit), N-Terminus antibody is suitable for immunoprecipitation and western blot at a dilution of 1:1,000-1:2,000 using rat brain lysate.

Biochem/physiol Actions

GABA is synthesized primarily from glutamate by glutamate decarboxylase (GAD). It is triggered by binding to its ligand-gated chloride channels, GABA(A) and GABA(C) as well as metabotropic receptor GABA(B). Along with neural development, it is also involved in a wide variety of physiological functions in tissues and organs outside the brain.

Physical form

Solution in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

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Storage Class Code

10 - Combustible liquids

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
094K1183

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Richard W Olsen et al.
Neuropharmacology, 56(1), 141-148 (2008-09-02)
This mini-review attempts to update experimental evidence on the existence of GABA(A) receptor pharmacological subtypes and to produce a list of those native receptors that exist. GABA(A) receptors are chloride channels that mediate inhibitory neurotransmission. They are members of the
U Rudolph et al.
Trends in pharmacological sciences, 22(4), 188-194 (2001-04-03)
The enhancement of GABA-mediated synaptic transmission underlies the pharmacotherapy of various neurological and psychiatric disorders. GABA(A) receptors are pluripotent drug targets that display an extraordinary structural heterogeneity: they are assembled from a repertoire of at least 18 subunits (alpha1-6, beta1-3
Structure and pharmacology of vertebrate GABAA receptor subtypes.
P J Whiting et al.
International review of neurobiology, 38, 95-138 (1995-01-01)
H Möhler et al.
Current opinion in pharmacology, 1(1), 22-25 (2001-11-20)
The GABA(A) receptor is a pluripotent drug target mediating anxiolytic, sedative, anticonvulsant, muscle relaxant and amnesic activity. These drug actions have now been attributed to defined receptor subtypes. Thus, precise guidelines are available for the development of novel drugs with
Masahito Watanabe et al.
International review of cytology, 213, 1-47 (2002-02-12)
Gamma-aminobutyrate (GABA) is a major inhibitory neurotransmitter in the adult mammalian brain. GABA is also considered to be a multifunctional molecule that has different situational functions in the central nervous system, the peripheral nervous system, and in some nonneuronal tissues.

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