G0774
Glycerokinase from Bacillus stearothermophilus
buffered aqueous solution, ≥75 units/mg protein (biuret)
Synonym(s):
ATP:glycerol 3-phosphotransferase, Glycerol Kinase
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About This Item
CAS Number:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-862-0
NACRES:
NA.54
MDL number:
EC Number:
Specific activity:
≥75 units/mg protein (biuret)
Assay:
≥5.0 mg protein/mL (biuret)
Biological source:
Bacillus sp. (Bacillus steaarothermophilus)
biological source
Bacillus sp. (Bacillus steaarothermophilus)
Quality Segment
assay
≥5.0 mg protein/mL (biuret)
form
buffered aqueous solution
specific activity
≥75 units/mg protein (biuret)
storage condition
dry at room temperature
color
beige
application(s)
life science and biopharma
storage temp.
2-8°C
Gene Information
Bacillus sp. ... glpK(89613566)
General description
Research area: Cell Signaling
Glycerol kinase is encoded by the GK gene on chromosome Xp21.2. Glycerol kinase is predominantly active in the liver.
Glycerol kinase is encoded by the GK gene on chromosome Xp21.2. Glycerol kinase is predominantly active in the liver.
Application
Biochem/physiol Actions
Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate.Oxidized glycerol-3-phosphate results in dihydroxyacetone phosphate which enters either glycolysis or gluconeogenesis. Elevated glycerol is observed in glycerol kinase deficiency leading to pseudohypertriglyceridemia.
Physical form
Stabilized solution in Tris buffer, pH 7.3
Other Notes
One unit will convert 1.0 μmole of glycerol and ATP to L -α-glycerophosphate and ADP per min at pH 9.8 at 25 °C in a coupled system with PK/LDH.
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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