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G115000

DMT-dG(dmf) Phosphoramidite

Name: DMT-dG(dmf) Phosphoramidite
Brand: SIGMA

≥99.0% (reversed phase HPLC)

Synonym(s):

DMT-dG(dmf) Amidite, N-[(dimethylamino)methylene]-5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-deoxyguanosine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite], N2-Dimethylformamidine-5′-O-(4,4′-dimethoxytrityl)-2′-deoxyguanosine-3′-O-[O-(2-cyanoethyl)-N,N′-diisopropylphosphoramidite]

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About This Item

Empirical Formula (Hill Notation):

C₄₃H₅₃N₈O₇P

CAS Number:

330628-04-1

Molecular Weight:

824.90

NACRES:

NA.51

PubChem Substance ID:

329799820

UNSPSC Code:

12352200

MDL number:

MFCD00797420

Assay:

≥99% (³¹P-NMR), ≥99.0% (reversed phase HPLC)

Form:

powder

Storage temp.:

-10 to -25°C

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Properties

type

for DNA synthesis

Quality Level

200

product line

Proligo Reagents

assay

≥99% (³¹P-NMR), ≥99.0% (reversed phase HPLC)

form

powder

mol wt

824.90 g/mol

technique(s)

oligo synthesis: suitable

color

white to off-white

λ

conforms (UV/VIS Identity)

nucleoside profile

base: deoxyguanosine
base protecting group: DMF
2' protecting group: none
5' protecting group: DMT
deprotection: fast

storage temp.

-10 to -25°C

SMILES string

COc1ccc(cc1)C(OC[C@H]2O[C@H](C[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)n3cnc4C(=O)NC(\N=C/N(C)C)=Nc34)(c5ccccc5)c6ccc(OC)cc6

InChI

1S/C43H53N8O7P/c1-29(2)51(30(3)4)59(56-24-12-23-44)58-36-25-38(50-28-45-39-40(50)47-42(48-41(39)52)46-27-49(5)6)57-37(36)26-55-43(31-13-10-9-11-14-31,32-15-19-34(53-7)20-16-32)33-17-21-35(54-8)22-18-33/h9-11,13-22,27-30,36-38H,12,24-26H2,1-8H3,(H,47,48,52)/b46-27-/t36-,37+,38+,59?/m0/s1

InChI key

YRQAXTCBMPFGAN-UJASEYITSA-N

Description

General description

DMT-dG(dmf) Phosphoramidite belongs to fast deprotection phosphoramidites where the deprotection step of automated oligonucleotide synthesis is integral to synthesis time and final product quality. Changing the dG protecting group to the dimethylformamidine (dmf) baseprotecting group enables the rapid synthesis of high-purity, high-yield oligonucleotide, thus increasing the efficiency of high-throughput production.

Features and Benefits

Key Features of dG(dmf):

dG(dmf) is deprotected faster than the conventional dG(ib): the deprotection time in concentrated ammonia is reduced to 2 hours at 55 °C or 1 hour at 65 °C
The dG(dmf)-monomer is especially suitable for G-rich sequences: incomplete deprotection is greatly reduced in comparison with the conventional dG(ib)-monomer
dG(dmf)-phosphoramidite is as stable in solution as the standard dA(bz)-, dC(bz)- and dT-phosphoramidites
dG(dmf)-phosphoramidite can directly substitute for dG(ib)-phosphoramidite
No change is required in the reagents commonly used for DNA synthesis (Except a low concentration iodine oxidizer i.e., 0.02 M in iodine, should be employed)

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Global Trade Item Number

SKU	GTIN
G115000-1KG	04061833444740
G115000-6X20G	04061833651810
G115000-100G	04061833651803
G115000-500G	04061833502990
G115000-BIO	04061822545755
G115000-C	04061826703274
G115000-6X5G	04061833368800
G115000-6X10G	04061833401972