G2513
β-Galactosidase from Escherichia coli
Grade X, ammonium sulfate suspension, ≥250 units/mg protein (biuret)
Synonym(s):
β-D-Galactoside galactohydrolase, Lactase
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About This Item
CAS Number:
UNSPSC Code:
12352204
EC Number:
232-864-1
MDL number:
type
Grade X
form
ammonium sulfate suspension
specific activity
≥250 units/mg protein (biuret)
storage temp.
2-8°C
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General description
Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)
Biochem/physiol Actions
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
Physical form
Suspension in 1.7 M (NH4)2SO4, 10 mM Tris buffer salts and 10 mM magnesium chloride, pH 7.3
Other Notes
One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.
wgk
WGK 3
Storage Class
13 - Non Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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