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Merck
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G2921

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

BioUltra, recombinant, expressed in E. coli, ammonium sulfate suspension, ≥95% (SDS-PAGE), ≥550 units/mg protein (biuret)

Synonym(s):

G-6-P-DH

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About This Item

CAS Number:
9001-40-5
UNSPSC Code:
12352204
MDL number:
MFCD00081656
EC Number:
1.1.1.49 (BRENDA, IUBMB)
Specific activity:
≥550 units/mg protein (biuret)
Assay:
≥95% (SDS-PAGE)
Recombinant:
expressed in E. coli

Key Documents

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recombinant

expressed in E. coli

product line

BioUltra

assay

≥95% (SDS-PAGE)

form

ammonium sulfate suspension

specific activity

≥550 units/mg protein (biuret)

foreign activity

Creatine Phosphokinase, Phosphoglucose isomerase, lactic dehydrogenase, glutathione reductase, NADH oxidase, NADPH oxidase, phosphoglucomutase, 6-phosphogluconic dehydrogenase, hexokinase ≤0.001%, Myokinase ≤0.005%

storage temp.

2-8°C

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General description

Glucose-6-phosphate dehydrogenase (G6PD) is an X-linked essential enzyme that protects cells from oxidative stress, particularly red blood cells.

Application

Glucose-6-phosphate dehydrogenase was used to study atypical glycolysis in Clostridium thermocellum.

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.
Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Physical form

Supplied in 3.2M ammonium sulfate containing 50mM Tris and 1mM magnesium chloride, pH 7.5

Other Notes

One unit will oxidize 1.0 μmole of D-glucose-6-phosphate to 6-phospho-D-gluconate per minute in the presence of NAD at pH 7.8 at 30°C

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

常规特殊物品
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Certificates of Analysis (COA)

Lot/Batch Number
107K7700

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Jilai Zhou et al.
Applied and environmental microbiology, 79(9), 3000-3008 (2013-02-26)
Cofactor specificities of glycolytic enzymes in Clostridium thermocellum were studied with cellobiose-grown cells from batch cultures. Intracellular glucose was phosphorylated by glucokinase using GTP rather than ATP. Although phosphofructokinase typically uses ATP as a phosphoryl donor, we found only pyrophosphate
Signs of endotracheal intubation in a field setting.
R D Stewart et al.
Annals of emergency medicine, 14(3), 276-277 (1985-03-01)
L Zuluaga et al.
Experimental parasitology, 133(1), 114-120 (2012-11-28)
Dehydroepiandrosterone (DHEA) inhibits glucose 6-phosphate dehydrogenase (G6PDH) of different species and may potentially decrease intracellular glutathione. Therefore, it can have and enhance anti-parasitic action against Plasmodium spp. We evaluated the antiplasmodial activity and the interaction of DHEA with several antimalarial
Clara Slade Oliveira et al.
Reproduction (Cambridge, England), 145(1), 9-17 (2012-10-30)
During initial development, both X chromosomes are active in females, and one of them must be silenced at the appropriate time in order to dosage compensate their gene expression levels to male counterparts. Silencing involves epigenetic mechanisms, including histone deacetylation.
Jie Liu et al.
Plant cell reports, 32(3), 415-429 (2012-12-13)
KEY MESSAGE : Two soybean cultivars showed markedly different drought tolerance. G6PDH plays a central role in the process of H ( 2 ) O ( 2 ) regulated GR, DHAR, and MDHAR activities to maintain GSH and Asc levels.
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