G3144
L-Glutamine Synthetase from Escherichia coli W, ATCC No. 9637
Protein ≥30 % by biuret, lyophilized powder
Synonym(s):
L-Glutamate: ammonia ligase (ADP-forming)
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.26
Pricing and availability is not currently available.
form
lyophilized powder
specific activity
100-400 units/mg protein
composition
Protein, ≥30% biuret
solubility
H2O: soluble 0.95-1.05 mg/mL, clear to slightly hazy
UniProt accession no.
storage temp.
−20°C
Gene Information
Escherichia coli K12 ... glnA(948370)
Application
L-Glutamine synthetase may be used for the purification of proteases from Escherichia coli.[1]
Biochem/physiol Actions
Degradative enzyme for glutamic acid
L-glutamine synthetase catalyzes the condensation of L-glutamate and ammonia to L-glutamine[2]. It is a degradative enzyme for glutamic acid.
Unit Definition
One unit will convert 1.0 μmole of L-glutamate to L-glutamine in 15 min at pH 7.1 at 37 °C.
Physical form
Lyophilized powder containing buffer salts and stabilizer
Preparation Note
Grown in medium containing glucose and NH4Cl
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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