Key Documents

View All Documentation

G3670

Galectin-8 from rat

Name: Galectin-8 from rat
Brand: SIGMA

recombinant, expressed in E. coli, ≥90% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

Gal-8, Po66 carbohydrate-binding protein, Prostate carcinoma tumor antigen 1

Select a Size

Change View

About This Item

UNSPSC Code:

12352202

MDL number:

MFCD07785205

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

biological source

rat

recombinant

expressed in E. coli

assay

≥90% (SDS-PAGE)

form

buffered aqueous glycerol solution

mol wt

protein 34 kDa

packaging

pkg of 100 μg

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

Q62665

shipped in

dry ice

storage temp.

−20°C

Gene Information

rat ... Lgals8(116641)

General description

Galectin-8 is one of the β-galactoside-binding lectins called galectins, which reside in the cytosol before being released in a signal sequence-independent pathway.

Biochem/physiol Actions

Galectin-8 acts as a physiological modulator of cell adhesion and cellular growth, and may be involved in neoplastic transformation.

Galectin-8 functions as a danger receptor and thus, prevents the proliferation of Salmonella. It detects host glycans found on the surface of damaged Salmonella-containing vacuoles. It also maintains the endo-lysosomal integrity. This protein prevents the adhesion of human carcinoma cells to integrin-ligand coated plates. This results in induction of apoptosis.

Galectin-8 is a family member of animal lectins which selectively binds β-galactoside residues. It is a widely expressed 34 kDa protein, which is secreted by many cell types. Galectin-8 is made of two homologous regions, each having a single carbohydrate recognition domain (CRD), linked by a short peptide. Galectin-8 acts as a physiological modulator of cell adhesion and cellular growth, and may be involved in neoplastic transformation.

Physical form

Solution containing 20 mM Tris, pH 7.4, 1 mM DTT, 1 mM EDTA, and 30% glycerol.

Analysis Note

The biological activity is measured by its ability to agglutinate human red cells.

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

新产品

This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

025K4124

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Galectins are differentially expressed in supratentorial pilocytic astrocytomas, astrocytomas, anaplastic astrocytomas and glioblastomas, and significantly modulate tumor astrocyte migration.

I Camby et al.

Brain pathology (Zurich, Switzerland), 11(1), 12-26 (2001-01-06)

Galectins, a family of mammalian lectins with specificity to beta-galactosides, are involved in growth-regulatory mechanisms and cell adhesion. A relationship is assumed to exist between the levels of expression of galectins and the level of malignancy in human gliomas. A

Sialylation by β-galactoside α-2,6-sialyltransferase and N-glycans regulate cell adhesion and invasion in human anaplastic large cell lymphoma.

Osamu Suzuki et al.

International journal of oncology, 46(3), 973-980 (2015-01-13)

The interaction between cell surface glycans and extracellular matrix (ECM) including galectins is known to be closely associated with tumor cell adhesion, invasion and metastasis. We analyzed the roles of cell surface sialylation or glycosylation in galectin or ECM‑mediated cell

Galectin-8 functions as a matricellular modulator of cell adhesion.

Y Levy et al.

The Journal of biological chemistry, 276(33), 31285-31295 (2001-05-24)

The interaction of cells with the extracellular matrix regulates cell adhesion and motility. Here we demonstrate that different cell types adhere and spread when cultured in serum-free medium on immobilized galectin-8, a mammalian beta-galactoside-binding protein. At maximal doses, galectin-8 is

Galectin 8 targets damaged vesicles for autophagy to defend cells against bacterial invasion.

Teresa L M Thurston et al.

Nature, 482(7385), 414-418 (2012-01-17)

Autophagy defends the mammalian cytosol against bacterial infection. Efficient pathogen engulfment is mediated by cargo-selecting autophagy adaptors that rely on unidentified pattern-recognition or danger receptors to label invading pathogens as autophagy cargo, typically by polyubiquitin coating. Here we show in

Galectin-8 binding to integrins inhibits cell adhesion and induces apoptosis.

Y R Hadari et al.

Journal of cell science, 113 ( Pt 13), 2385-2397 (2000-06-15)

The interaction of cells with the extracellular matrix regulates cell adhesion, motility, growth, survival and differentiation through integrin-mediated signal transduction. Here we demonstrate that galectin-8, a secreted mammalian (beta)-galactoside binding protein, inhibits adhesion of human carcinoma (1299) cells to plates