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G3907

Glutathione−Agarose

Name: Glutathione−Agarose
Brand: SIGMA

set of 3 pre-packed columns (2.5 ml each), (1:1 suspension in a 0.5 M NaCl + 20% ethanol solution)

Synonym(s):

GSH-agarose, S-linked glutathione agarose

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About This Item

UNSPSC Code:

41106500

NACRES:

NA.56

MDL number:

MFCD00131200

Matrix:

cross-linked 4% beaded agarose

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Quality Level

200

form

(1:1 suspension in a 0.5 M NaCl + 20% ethanol solution)

analyte chemical class(es)

proteins (GST)

packaging

set of 3 pre-packed columns (2.5 ml each)

technique(s)

immunoprecipitation (IP): suitable, protein purification: suitable

matrix

cross-linked 4% beaded agarose

storage temp.

2-8°C

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Related Categories

Protein Biology

Protein Purification Chromatography

Protein Sample Prep

Recombinant/Fusion Tag Protein Purification

General description

The resin in these columns consists of glutathione attached through the sulfur to epoxy activated, 4% cross-linked beaded agarose resulting in a 12 atom spacer.

Application

Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing and highly selective purification of glutathione binding enzymes such as glutathione-S-transferase, glutathione peroxidase, and glyoxalase I.
The product was used in the design, synthesis and biological evaluation of new tryptamine and tetrahydro-β-carboline-based selective inhibitors of CDK4. It was also used to study Fascaplysin-inspired diindolyls as selective inhibitors of CDK4/cyclin D1.

Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing and highly selective purification of proteins containing glutathione binding sequences, such as Glutathione S-Transferase (GST), glutathione peroxidase and glyoxalase I.

Physical form

1:1 suspension in a 0.5 M NaCl + 20% ethanol solutionl

Other Notes

Suspension of Product No. G 4510

Storage Class

10 - Combustible liquids

flash_point_f

180.1 °F

flash_point_c

82.3 °C

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number

SLBR3849V

016K7056

074K7025

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Design, synthesis and biological evaluation of new tryptamine and tetrahydro-beta-carboline-based selective inhibitors of CDK4.

Paul R Jenkins et al.

Bioorganic & medicinal chemistry, 16(16), 7728-7739 (2008-07-25)

We present the design, synthesis and biological activity of a library of substituted (biphenylcarbonyl)-tryptamine and (biphenylcarbonyl)-tetrahydro-beta-carboline compounds related to the natural product fascaplysin, as novel inhibitors of CDK4/cyclin D1. We show all these molecules, prepared using the Suzuki-Miyaura reaction, being

Design, synthesis and biological activity of new CDK4-specific inhibitors, based on fascaplysin.

Carine Aubry et al.

Organic & biomolecular chemistry, 4(5), 787-801 (2006-02-24)

We present the design, synthesis, and biological activity of three classes of tryptamine derivatives, which are non-planar analogues of the toxic anti-cancer agent fascaplysin. We show these compounds to be selective inhibitors of CDK4 over CDK2, the most active compound

Sld2 binds to origin single-stranded DNA and stimulates DNA annealing.

Diane M Kanter et al.

Nucleic acids research, 39(7), 2580-2592 (2010-11-27)

Sld2 is essential for the initiation of DNA replication, but the mechanism underlying its role in replication is not fully understood. The S-phase cyclin dependent kinase (S-CDK) triggers the association of Sld2 with Dpb11, and a phosphomimetic mutation of Sld2

Methods for purification of glutathione peroxidase and related enzymes.

F Toribio et al.

Journal of chromatography. B, Biomedical applications, 684(1-2), 77-97 (1996-09-20)

The different preparative techniques and related analytical methods used for purification of glutathione peroxidase, glutathione transferase and glutathione reductase, described in papers published in the last ten years, have been reviewed in this article. Among the different purification techniques, chromatography

Extremes of clinical and enzymatic phenotypes in children with hyperinsulinism caused by glucokinase activating mutations.

Samir Sayed et al.

Diabetes, 58(6), 1419-1427 (2009-04-02)

Heterozygous activating mutations of glucokinase have been reported to cause hypoglycemia attributable to hyperinsulinism in a limited number of families. We report three children with de novo glucokinase hyperinsulinism mutations who displayed a spectrum of clinical phenotypes corresponding to marked

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Global Trade Item Number

SKU	GTIN
G3907-1SET	04061833630327

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