G4388
Glycerol 3-phosphate Oxidase from Streptococcus thermophilus
lyophilized powder, ≥35 units/mg solid, pH 7.0
Synonym(s):
sn-Glycerol 3-phosphate: oxygen 2-oxidoreductase, L-Glycerol 3-phosphate Oxidase
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About This Item
CAS Number:
UNSPSC Code:
12352204
EC Number:
232-932-0
MDL number:
form
lyophilized powder
specific activity
≥35 units/mg solid, pH 7.0
mol wt
131 kDa
shipped in
wet ice
storage temp.
−20°C
Application
GPO has been used for sensitive metabolite assays of starch and lipid synthesis, pyrophosphate, ATP, ADP, and most glycolytic intermediates in Arabidopsis seeds. GPO is part of the dihydroxyacetone phosphate:glycerol-3-phosphate cycle in the bloodstream form of Trypanosoma brucei.
Biochem/physiol Actions
Glycerol-3-phosphate oxidase catalyzes the conversion of sn-glycerol-3-phosphate to glycerone phosphate and peroxide.
Other Notes
One unit will oxidize 1.0 μmole of L-glycerol 3-phosphate to dihydroxyacetone phosphate with the formation of H2O2 per min at pH 7.0 at 37 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Localization of glycerol-3-phosphate oxidase in the mitochondrion and particulate NAD+-linked glycerol-3-phosphate dehydrogenase in the microbodies of the bloodstream form to Trypanosoma brucei.
F R Opperdoes et al.
European journal of biochemistry, 76(1), 29-39 (1977-06-01)
Shu-Yi Hsu et al.
Sensors (Basel, Switzerland), 10(6), 5758-5773 (2010-01-01)
A single use, disposable iridium-nano particle contained biosensor had been developed for the determination of diglyceride (DG). In this study hydrogen peroxide, formed through the enzymatic breakdown of DG via lipase, glycerol kinase and glycerol 3-phosphate oxidase, was electrochemically oxidized
Tatsuya Kishimoto et al.
Clinica chimica acta; international journal of clinical chemistry, 333(1), 59-67 (2003-06-18)
Several methods for measuring concentrations of lysophosphatidic acid (LPA), a lipid mediator, have been reported to date. However, these methods are not routinely used because most of them require specialized instrument and a complicated protocol. We developed a novel LPA
C S Pundir et al.
Clinical biochemistry, 43(4-5), 467-472 (2009-12-29)
A method is described for construction of an amperometric triglyceride (TG) biosensor using PVA membrane bound enzymes. A mixture of commercial lipase, glycerol kinase, glycerol-3-phosphate oxidase and horseradish peroxidase was co-immobilized onto polyvinyl alcohol (PVA) membrane through glutaraldehyde coupling. The
Alain Bizzini et al.
Journal of bacteriology, 192(3), 779-785 (2009-12-08)
Enterococcus faecalis is equipped with two pathways of glycerol dissimilation. Glycerol can either first be phosphorylated by glycerol kinase and then oxidized by glycerol-3-phosphate oxidase (the glpK pathway) or first be oxidized by glycerol dehydrogenase and then phosphorylated by dihydroxyacetone
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