G4388
Glycerol 3-phosphate Oxidase from Streptococcus thermophilus
lyophilized powder, ≥35 units/mg solid, pH 7.0
Synonym(s):
sn-Glycerol 3-phosphate: oxygen 2-oxidoreductase, L-Glycerol 3-phosphate Oxidase
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About This Item
CAS Number:
UNSPSC Code:
12352204
EC Number:
232-932-0
MDL number:
form
lyophilized powder
specific activity
≥35 units/mg solid, pH 7.0
mol wt
131 kDa
shipped in
wet ice
storage temp.
−20°C
Application
GPO has been used for sensitive metabolite assays of starch and lipid synthesis, pyrophosphate, ATP, ADP, and most glycolytic intermediates in Arabidopsis seeds. GPO is part of the dihydroxyacetone phosphate:glycerol-3-phosphate cycle in the bloodstream form of Trypanosoma brucei.
Biochem/physiol Actions
Glycerol-3-phosphate oxidase catalyzes the conversion of sn-glycerol-3-phosphate to glycerone phosphate and peroxide.
Other Notes
One unit will oxidize 1.0 μmole of L-glycerol 3-phosphate to dihydroxyacetone phosphate with the formation of H2O2 per min at pH 7.0 at 37 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Localization of glycerol-3-phosphate oxidase in the mitochondrion and particulate NAD+-linked glycerol-3-phosphate dehydrogenase in the microbodies of the bloodstream form to Trypanosoma brucei.
F R Opperdoes et al.
European journal of biochemistry, 76(1), 29-39 (1977-06-01)
Glycerol metabolism in yeasts. Pathways of utilization and production.
C Gancedo et al.
European journal of biochemistry, 5(2), 165-172 (1968-07-01)
Jagriti Narang et al.
International journal of biological macromolecules, 47(5), 691-695 (2010-09-14)
A mixture of commercial lipase, glycerol kinase and glycerol-3-phosphate oxidase was co-immobilized onto egg shell membrane through covalent coupling. A method is described for fabrication of a triglyceride (TG) biosensor using egg shell membrane bound enzymes. The biosensor measured current
Minakshi et al.
Indian journal of biochemistry & biophysics, 45(2), 111-115 (2008-04-01)
Commercial lipase, glycerol kinase (GK), glycerol-3-phosphate oxidase (GPO) and peroxidase (POD) have been co-immobilized covalently on to arylamine glass beads affixed on a plastic strip through diazotization with a conjugation yield of 89.1 mg/g support and 64.1% retention of specific
Claudine Hames et al.
Journal of bacteriology, 191(3), 747-753 (2008-11-26)
Glycerol is one of the few carbon sources that can be utilized by Mycoplasma pneumoniae. Glycerol metabolism involves uptake by facilitated diffusion, phosphorylation, and the oxidation of glycerol 3-phosphate to dihydroxyacetone phosphate, a glycolytic intermediate. We have analyzed the expression
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