G4388
Glycerol 3-phosphate Oxidase from Streptococcus thermophilus
lyophilized powder, ≥35 units/mg solid, pH 7.0
Synonym(s):
sn-Glycerol 3-phosphate: oxygen 2-oxidoreductase, L-Glycerol 3-phosphate Oxidase
form
lyophilized powder
specific activity
≥35 units/mg solid, pH 7.0
mol wt
131 kDa
shipped in
wet ice
storage temp.
−20°C
Application
GPO has been used for sensitive metabolite assays of starch and lipid synthesis, pyrophosphate, ATP, ADP, and most glycolytic intermediates in Arabidopsis seeds. GPO is part of the dihydroxyacetone phosphate:glycerol-3-phosphate cycle in the bloodstream form of Trypanosoma brucei.
Biochem/physiol Actions
Glycerol-3-phosphate oxidase catalyzes the conversion of sn-glycerol-3-phosphate to glycerone phosphate and peroxide.
Other Notes
One unit will oxidize 1.0 μmole of L-glycerol 3-phosphate to dihydroxyacetone phosphate with the formation of H2O2 per min at pH 7.0 at 37 °C.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Localization of glycerol-3-phosphate oxidase in the mitochondrion and particulate NAD+-linked glycerol-3-phosphate dehydrogenase in the microbodies of the bloodstream form to Trypanosoma brucei.
F R Opperdoes et al.
European journal of biochemistry, 76(1), 29-39 (1977-06-01)
Shao-Peng Chen et al.
The Analyst, 135(11), 2979-2986 (2010-09-30)
In this paper an enzyme-carrier-based microfluidic chip coupled with a gold nanoband microelectrode as electrochemical detector for Triglyceride (TG) determination was developed by co-immobilized lipase, Glycerokinase (GK) and glycerol-3-phosphate oxidase (GPOx) on chitosan/Fe(3)O(4) composite nanoparticles with a shell-core structure, which
Richard M Denton
Biochimica et biophysica acta, 1787(11), 1309-1316 (2009-05-06)
Studies in Bristol in the 1960s and 1970s, led to the recognition that four mitochondrial dehydrogenases are activated by calcium ions. These are FAD-glycerol phosphate dehydrogenase, pyruvate dehydrogenase, NAD-isocitrate dehydrogenase and oxoglutarate dehydrogenase. FAD-glycerol phosphate dehydrogenase is located on the
Sebastian R Schmidl et al.
PLoS pathogens, 7(9), e1002263-e1002263 (2011-10-04)
Mycoplasma pneumoniae is a causative agent of atypical pneumonia. The formation of hydrogen peroxide, a product of glycerol metabolism, is essential for host cell cytotoxicity. Phosphatidylcholine is the major carbon source available on lung epithelia, and its utilization requires the
Yves Gibon et al.
The Plant journal : for cell and molecular biology, 30(2), 221-235 (2002-05-10)
Metabolite assays are required to characterise how metabolism changes between genotypes during development and in response to environmental perturbations. They provide a springboard to identify important regulatory sites and investigate the underlying mechanisms. Due to their small size, Arabidopsis seeds
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