G4510
Glutathione−Agarose
lyophilized powder
Synonym(s):
Glutathione-Agarose Resin, GSH-agarose, S-linked glutathione agarose
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About This Item
Form:
lyophilized powder
form
lyophilized powder
Quality Level
analyte chemical class(es)
proteins (GST)
extent of labeling
≥10 μmol per mL gel
technique(s)
immunoprecipitation (IP): suitable, protein purification: suitable
matrix
4% cross-linked beaded agarose
matrix activation
epoxy
matrix attachment
sulfur
matrix spacer
12 atoms (10 carbon)
storage temp.
−20°C
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General description
Glutathione S-transferase (GST) is a protein with a molecular weight of 26 KDa that naturally occurs in eukaryotic cells. It belongs to the family of Phase II detoxification enzymes. Glutathione-Agarose consists of glutathione attached through its sulfur to epoxy-activated 4% cross-linked beaded agarose, resulting in a 12-atom (10-carbon) spacer. It is provided as a lyophilized powder stabilized with lactose.
Application
Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing and highly selective purification of proteins containing glutathione binding sequences, such as Glutathione S-Transferase (GST), glutathione peroxidase and glyoxalase I.
Glutathione-Agarose has been used:
- to purify glutathione S-transferase (GST)-fused protein for generating polyclonal antibody M4P
- to incubate supernatant fractions for the purification of full-length p53 (aa 1-393) and its DNA-binding domain (aa 94-288)
- in affinity purification of the fusion protein
Biochem/physiol Actions
Glutathione S-transferases (GSTs), a superfamily of multifunctional detoxification isoenzymes, have a significant role in cellular signaling. GSTs are emerging as promising therapeutic targets due to the overexpression of specific isozymes in various tumors and their potential involvement in the development of other diseases such as neurodegenerative diseases, multiple sclerosis, and asthma.
Features and Benefits
- Offers rapid, mild, non-denaturing, and highly selective purification of glutathione-binding enzymes.
- Has a shelf life of two years if stored properly desiccated at -20 °C.
- Binding capacity: 5-10 mg glutathione S-transferase per mL resin
Physical form
Lyophilized powder stabilized with lactose
Preparation Note
One g powder swells to ≥10 ml of gel
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
动植物源性产品
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Related Content
利用亲和力、GST pull-down、TAP 和共免疫沉淀方法，通过Pull-Down研究体外蛋白质互作。
Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| G4510-50ML | 04061833631485 |
| G4510-10ML | 04061833631447 |
| G4510-1ML | 04061833631454 |
| G4510-5ML | 04061833631546 |
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