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Merck
CN

G5259

Glyoxylate reductase from Spinacia oleracea (spinach)

ammonium sulfate suspension, ~50 units/mg protein (biuret)

Synonym(s):

Glycolate dehydrogenase, Glycolate:NAD+ oxidoreductase, Hydroxypyruvate reductase

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About This Item

CAS Number:
UNSPSC Code:
12352204
EC Number:
232-828-5
MDL number:
EC Number:
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ammonium sulfate suspension

specific activity

~50 units/mg protein (biuret)

storage temp.

2-8°C

Biochem/physiol Actions

Contains associated glycerate dehydrogenase activity
Glycerate Dehydrogenase Activity: 100-300 units per mg protein (biuret); one unit will convert 1.0 μmole of hydroxypyruvate to D-glycerate per min at pH 6.0 at 25°C.

Physical form

Suspension in 3.0 M (NH4)2SO4, 0.01 M KH2PO4, pH 7.0

Other Notes

One unit will convert 1.0 μmole of glyoxylate to glycolate per min at pH 6.4 at 25°C.

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Takeshi Shinoda et al.
Biochemical and biophysical research communications, 355(3), 782-787 (2007-02-27)
A Glu141Asn mutant Paracoccus sp. 12-A formate dehydrogenase catalyzes marked glyoxylate reduction. Additional replacement of the His332-Gln313 pair with His-Glu, which is a consensus acid/base catalyst in D-hydroxyacid dehydrogenases, further improved the catalytic activity of the enzyme as to glyoxylate
John Knight et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 21(8), 2292-2295 (2006-04-07)
Primary hyperoxaluria type 2 (PH2) is a rare monogenic disorder characterized by an elevated urinary excretion of oxalate. Increased oxalate excretion in PH2 patients can cause nephrolithiasis and nephrocalcinosis, and can, in some cases, result in renal failure and systemic
Is liver analysis still required for the diagnosis of primary hyperoxaluria type 2?
Gill Rumsby
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 21(8), 2063-2064 (2006-07-06)
Eija Rintala et al.
Yeast (Chichester, England), 24(2), 129-136 (2006-12-19)
The enzyme glyoxylate reductase reversibly reduces glyoxylate to glycolate, or alternatively hydroxypyruvate to D-glycerate, using either NADPH or NADH as a co-factor. The enzyme has multiple metabolic roles in different organisms. In this paper we show that GOR1 (ORF YNL274c)
Wendy L Allan et al.
The Biochemical journal, 423(1), 15-22 (2009-09-11)
Molecular modelling suggests that a group of proteins in plants known as the beta-hydroxyacid dehydrogenases, or the hydroxyisobutyrate dehydrogenase superfamily, includes enzymes that reduce succinic semialdehyde and glyoxylate to gamma-hydroxybutyrate and glycolate respectively. Recent biochemical and expression studies reveal that

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