G5758
Glucose-6-Phosphatase from rabbit liver
≥0.05 units/mg protein, Protein ~30 % (balance mostly sucrose)
Synonym(s):
D-Glucose-6-Phosphate Phosphohydrolase
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
form
powder with sucrose
specific activity
≥0.05 units/mg protein
composition
Protein, ~30% (balance mostly sucrose)
storage temp.
−20°C
General description
Glucose-6-phosphatase is a multicomponent protein complex comprising of glucose-6-phosphate transporter (G6PT) and a catalytic unit (G6PC). Liver and kidney cells show predominant expression of glucose-6-phosphatase.
Application
Glucose-6-Phosphatase from rabbit liver has been used in glucose 6-phosphatase inhibitory assay of crude C. anisata leaf extracts and methanolic leaf and bark extracts of Dolichandrone atrovirens.
Glucose-6-Phosphatase was used to study the effects of Clausena anisata (Wild hook leaf) extracts on selected diabetes-related metabolizing enzymes.
Biochem/physiol Actions
Glucose-6-Phosphatase is crucial for regulating glucose homeostasis during starvation. Deficiency of glucose-6-phosphatase is implicated in glycogen storage disease type I (GSDI).
Glucose-6-phosphatase catalyzes the conversion of glucose-6-phosphate into glucose as the last step of gluconeogenesis.
Analysis Note
Crude microsomal preparation
Other Notes
It has been reported that glucose-6-phosphatase, inorganic pyrophosphatase, and pyrophosphate glucose phosphotransferase are associated with a single protein that catalyzes three different reactions:
a) glucose 6-phosphate + H2O → glucose + Pi
b) PPi + H2O → 2 Pi
c) glucose + PPi or nucleoside-DP or -TP → glucose 6-phosphate + Pi or nucleoside-MP.
a) glucose 6-phosphate + H2O → glucose + Pi
b) PPi + H2O → 2 Pi
c) glucose + PPi or nucleoside-DP or -TP → glucose 6-phosphate + Pi or nucleoside-MP.
One unit will release 1.0 μmole of inorganic phosphate from glucose 6-phosphate per min at pH 6.5 at 37 °C.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Seung Won Ahn et al.
The Journal of biological chemistry, 287(50), 41875-41887 (2012-10-18)
Cyclic AMP (cAMP) induces steroidogenic enzyme gene expression and stimulates testosterone production in Leydig cells. Phosphoenolpyruvate carboxykinase (PEPCK) is expressed in Leydig cells, but its role has not been defined. In this study, we found that PEPCK and glucose-6-phosphatase (Glc-6-Pase)
Sua In et al.
Neuroscience letters, 530(2), 166-171 (2012-10-10)
Molecular mechanisms of body weight control have been discovered recently and much research focuses on the hypothalamic regulation of food intake and the hepatic regulation of glucose utility. We previously reported that postnatal nicotinamide treatment reduced brain dopamine and body
Yan Wang et al.
Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics, 14(11), 856-858 (2012-11-14)
To investigate the mutation of glucose-6-phosphatase gene (G6PC gene) in a patient with glycogen storage disease Ⅰa. PCR was used to amplify all five exons of G6PC gene. The PCR products were directly sequenced to detect the mutations. A heterozygous
Glucotoxicity Induces Glucose-6-Phosphatase Catalytic Unit Expression by Acting on the Interaction of Hypoxia Inducible Factor-1alpha With cAMP-Responsive Element-Binding Protein-Binding Protein
Gautier-Stein A, et al.
Diabetes, DB_110986-DB_110986 (2012)
Bidur Bhandary et al.
Biological & pharmaceutical bulletin, 35(11), 1907-1913 (2012-11-06)
Hyperlipidemia is a major contributor for atherosclerosis and hypolipidemic drugs such as statin are highly prescribed to treat elevated lipid level in plasma. Rubus coreanus, which is widely cultivated in south eastern Asia, have been reported to show significant cholesterol
Protocols
To measure glucose-6-phosphatase activity, the Taussky-Shorr method is used. This method is a spectrophotometric stop-rate determination assay that is measured at 660 nm.
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