G5758
Glucose-6-Phosphatase from rabbit liver
≥0.05 units/mg protein, Protein ~30 % (balance mostly sucrose)
Synonym(s):
D-Glucose-6-Phosphate Phosphohydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
form
powder with sucrose
specific activity
≥0.05 units/mg protein
composition
Protein, ~30% (balance mostly sucrose)
storage temp.
−20°C
General description
Glucose-6-phosphatase is a multicomponent protein complex comprising of glucose-6-phosphate transporter (G6PT) and a catalytic unit (G6PC). Liver and kidney cells show predominant expression of glucose-6-phosphatase.
Application
Glucose-6-Phosphatase from rabbit liver has been used in glucose 6-phosphatase inhibitory assay of crude C. anisata leaf extracts and methanolic leaf and bark extracts of Dolichandrone atrovirens.
Glucose-6-Phosphatase was used to study the effects of Clausena anisata (Wild hook leaf) extracts on selected diabetes-related metabolizing enzymes.
Biochem/physiol Actions
Glucose-6-Phosphatase is crucial for regulating glucose homeostasis during starvation. Deficiency of glucose-6-phosphatase is implicated in glycogen storage disease type I (GSDI).
Glucose-6-phosphatase catalyzes the conversion of glucose-6-phosphate into glucose as the last step of gluconeogenesis.
Analysis Note
Crude microsomal preparation
Other Notes
It has been reported that glucose-6-phosphatase, inorganic pyrophosphatase, and pyrophosphate glucose phosphotransferase are associated with a single protein that catalyzes three different reactions:
a) glucose 6-phosphate + H2O → glucose + Pi
b) PPi + H2O → 2 Pi
c) glucose + PPi or nucleoside-DP or -TP → glucose 6-phosphate + Pi or nucleoside-MP.
a) glucose 6-phosphate + H2O → glucose + Pi
b) PPi + H2O → 2 Pi
c) glucose + PPi or nucleoside-DP or -TP → glucose 6-phosphate + Pi or nucleoside-MP.
One unit will release 1.0 μmole of inorganic phosphate from glucose 6-phosphate per min at pH 6.5 at 37 °C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Glucose-6-phosphatase catalytic subunit gene family
Hutton JC and O'Brien RM
The Journal of Biological Chemistry, 284(43), 29241-29245 (2009)
Mohd Aidil Azahar et al.
Asian Pacific journal of tropical medicine, 5(11), 875-881 (2012-11-14)
To investigate the hypoglycemic effect of the aqueous extract of Octomeles sumatrana (O. sumatrana) (OS) in streptozotocin-induced diabetic rats (STZ) and its molecular mechanisms. Diabetes was induced by intraperitoneal (i.p.) injection of streptozotocin (55 mg/kg) in to male Sprague-Dawley rats.
Emile van Schaftingen et al.
The Biochemical journal, 362(Pt 3), 513-532 (2002-03-07)
Glucose-6-phosphatase (G6Pase), an enzyme found mainly in the liver and the kidneys, plays the important role of providing glucose during starvation. Unlike most phosphatases acting on water-soluble compounds, it is a membrane-bound enzyme, being associated with the endoplasmic reticulum. In
Loranne Agius
Biochemical pharmacology, 85(3), 306-312 (2012-10-02)
In population studies hepatic steatosis in subjects with Non-alcoholic fatty liver disease (NAFLD) is strongly associated with insulin resistance. This association has encouraged debate whether hepatic steatosis is the cause or the consequence of hepatic insulin resistance? Although genome-wide studies
The effects of Clausena anisata (Wild) Hook leaf extracts on selected diabetic related metabolizing enzymes
Mogale MA, et al.
Journal of Medicinal Plants Research, 6(25), 4200-4207 (2012)
Protocols
To measure glucose-6-phosphatase activity, the Taussky-Shorr method is used. This method is a spectrophotometric stop-rate determination assay that is measured at 660 nm.
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