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G5921

L-Glutamate Oxidase from Streptomyces sp.

recombinant, expressed in E. coli, lyophilized powder, ≥5.0 units/mg solid

Synonym(s):

L-Glutamate:oxygen oxidoreductase (deaminating)

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About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥5.0 units/mg solid
Recombinant:
expressed in E. coli
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recombinant

expressed in E. coli

Quality Level

form

lyophilized powder

specific activity

≥5.0 units/mg solid

storage temp.

2-8°C

General description

Glutamate is a non-essential amino acid. It acts as a primary excitatory neurotransmitter. In major trauma, major surgery, sepsis and bone marrow transplantation, glutamate functions as an essential amino acid.

Application

L-Glutamate Oxidase from Streptomyces sp has been used to determine the enzyme activity of the immobilized glutamate oxidase (GOx). It has also been used as a component of imaging buffer for stochastic optical reconstruction microscopy (STORM) imaging.

Biochem/physiol Actions

L-glutamate oxidase catalyzes the conversion of L-glutamate to 2-oxoglutarate.
Major enzyme in the synthesis or degradation of glutamic acid; transfers an amine to α-ketoglutaric acid to form L-glutamic acid or deamidates L-glutamic acid

Other Notes

One unit will form 1.0 μmole of α-ketoglutaric acid from L-glutamic acid per min at pH 7.4 at 30 °C.


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

常规特殊物品

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II. Glutamine and glutamate
Tapiero H, et al.
Biomedicine and Pharmacotherapy, 56(9), 446-457 (2002)
Purification and properties of a new enzyme, L-glutamate oxidase, from Streptomyces sp X-119-6 grown on wheat bran.
H. Yoshino
Agricultural and Biological Chemistry, 74, 1323-1328 (1983)
Stefania Digiovanni et al.
Scientific reports, 10(1), 10135-10135 (2020-06-25)
Reactive Intermediate Deaminase (Rid) protein superfamily includes eight families among which the RidA is conserved in all domains of life. RidA proteins accelerate the deamination of the reactive 2-aminoacrylate (2AA), an enamine produced by some pyridoxal phosphate (PLP)-dependent enzymes. 2AA



Global Trade Item Number

SKUGTIN
G5921-1UN04061833638682
G5921-5UN04061833638699