G6672
Anti-GGH (167-180) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Synonym(s):
Anti-γ-Glutamyl hydrolase precursor, Anti-GH
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About This Item
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
WB
Citations:
3
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~36 kDa
species reactivity
human
technique(s)
western blot: 1:500-1:2,000
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... GGH(8836)
General description
γ-Glutamyl hydrolase is a lysosomal and secreted glycoprotein that is abundantly expressed in liver and kidney. It may be used as a target for inhibition to enhance the therapeutic effect of antifolates and fluorouracil.
Immunogen
synthetic peptide corresponding to amino acids 167-180 of human GGH
Application
Anti-GGH antibody produced in rabbit is suitable for western blot analysis at a working dilution of 1:500-1:2000.
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.
Biochem/physiol Actions
GGH (γ-glutamyl hydrolase) encodes an enzyme that hydrolyzes the γ-glutamyl sidechains of antifolate and folate polyglutamates. Removal of γ-glutamyl residues from pteroylpoly-γ-glutamate yields pteroyl-α-glutamate (folic acid) and free glutamate.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Regulatory Information
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Related Content
J Galivan et al.
Seminars in oncology, 26(2 Suppl 6), 33-37 (1999-12-22)
Glutamyl hydrolase cleaves the poly-gamma-glutamate chain folate and antifolate poly-gamma-glutamates. Its cellular location is lysosomal with large amounts of the enzyme constitutively secreted. The highest levels of glutamyl hydrolase mRNA in humans is found in the liver and kidney. Baculovirus-expressed
J Galivan et al.
Pharmacology & therapeutics, 85(3), 207-215 (2000-03-31)
gamma-Glutamyl hydrolase (GH, EC 3.4.19.9) is a lysosomal and secreted glycoprotein that hydrolyzes the gamma-glutamyl tail of antifolate and folate polyglutamates. Tumor cells that have high levels of GH are inherently resistant to classical antifolates, and further resistance can be
R Yao et al.
Proceedings of the National Academy of Sciences of the United States of America, 93(19), 10134-10138 (1996-09-17)
A cDNA encoding human gamma-glutamyl hydrolase has been identified by searching an expressed sequence tag data base and using rat gamma-glutamyl hydrolase cDNA as the query sequence. The cDNA encodes a 318-amino acid protein of Mr 35,960. The deduced amino