G7775
Monoclonal Anti-Glycophorin C (β) antibody produced in mouse
clone E5, purified immunoglobulin
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About This Item
UNSPSC Code:
12352203
MDL number:
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
E5, monoclonal
species reactivity
human
technique(s)
agglutination assay: 1:50 using human erythrocytes, flow cytometry: suitable using bone marrow nucleated cells, indirect immunofluorescence: suitable using bone marrow nucleated cells, western blot: suitable using extracts of human red blood cell ghosts
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... GYPC(2995)
Immunogen
human thymus
Application
Monoclonal Anti-Glycophorin C (β) antibody produced in mouse is suitable for immunoblot analysis to examine the association of glycophorin C with HGP44 protein.
The antibody is suitable for:
The antibody is suitable for:
- agglutination assay at a working dilution of 1:50 using human erythrocytes
- flow cytometry using bone marrow nucleated cells
- indirect immunofluorescence using bone marrow nucleated cells
- western blot using extracts of human red blood cell ghosts
Biochem/physiol Actions
By immunoblotting, the antibody localizes specifically the Gerbich blood group antigen-related sialoglycoprotein β in extracts of human red blood cell ghosts. The antibody (also cited as clone no. 2C10) binds to 18% of bone marrow nucleated cells in smears and tissue preparations using immunofluorescent microscopy or flow cytometry.
Glycophorins (GP) are sialic acid-rich polypeptides (sialoglycoproteins) that are part of the erythrocyte membrane. They are denoted α, β, γ, δ based on the decreasing molar mass. Glycophorin C (GPC) is a minor red blood cell sialoglycoprotein. It has a molar mass of 35-38kDa. It plays a key role in maintaining the shape of erythrocytes by regulating the deformability and mechanical stability of red cells. It is usually found among the erythroid progenitors in early erythroid differentiation.
Physical form
Solution in 0.1 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Monoclonal antibodies against glycophorins and other glycoproteins.
D J Anstee et al.
Journal of immunogenetics, 17(4-5), 301-308 (1990-08-01)
Eiko Sakai et al.
Journal of bacteriology, 189(11), 3977-3986 (2007-03-27)
Porphyromonas gingivalis, an anaerobic gram-negative bacterium associated with chronic periodontitis, can agglutinate human erythrocytes. In general, hemagglutination can be considered the ability to adhere to host cells; however, P. gingivalis-mediated hemagglutination has special significance because heme markedly accelerates growth of
M J Telen et al.
Transfusion, 27(4), 309-314 (1987-07-01)
The minor red cell sialoglycoproteins--beta and gamma (also known as glycophorin C)--are believed to be important to the structural integrity of red cells. The absence of sialoglycoproteins alpha and delta, as seen in En(a-) and S-s-U- cells, respectively, results in
M J Telen et al.
Vox sanguinis, 52(3), 236-243 (1987-01-01)
Human erythrocyte membrane proteins express antigens which serve as markers for erythroid differentiation as well as targets for human blood group alloantibodies. We have produced and characterized a new panel of five monoclonal antibodies to erythrocyte membrane proteins. Three monoclonal
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Datasheet
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