Gene Transfer Methods: Introducing DNA Into Living Cells and Organisms

A critical aspect of the molecular revolution is that DNA frequently must be re-introduced into living cells to determine activity. The goal of this book is to compile a core set of numerous DNA transfer methods within a single volume. Chapters discuss DNA transfer into cells of bacterial, fungal, and mammalian origin, as well as into the germlines of flies, mice, and plants. Each chapter includes supplementary information such as common problems and specific troubleshooting suggestions. Progress and hurdles in the field of human gene therapy are presented.