G8511
β-Galactosidase from Escherichia coli
For use as a marker in SDS-PAGE
Synonym(s):
β-D-Galactoside galactohydrolase, Lactase
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About This Item
CAS Number:
UNSPSC Code:
12352200
NACRES:
NA.25
EC Number:
232-864-1
MDL number:
mol wt
~116 kDa
packaging
vial of 0.5 mg
storage temp.
2-8°C
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General description
Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)
Application
β-Galactosidase from Escherichia coli has been used as an internal standard in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). It has been used in the protein ladder mix for optimization of microfluidic western blotting assay.
Biochem/physiol Actions
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
Other Notes
One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Microfluidic Western blotting of low-molecular-mass proteins.
Gerver RE and Herr AE
Analytical Chemistry, 86, 10625-10632 (2014)
Quantitative determination of titin and nebulin in poultry meat by SDS-PAGE with an internal standard.
Tomaszewska-Gras J, et al.
Meat Science, 62, 61-66 (2002)
Scott W McPherson et al.
Journal of immunology (Baltimore, Md. : 1950), 190(10), 4971-4981 (2013-04-16)
Extrathymically derived regulatory T cells (iTregs) protect against autoimmunity to tissue-specific Ags. However, whether Ag-specific iTreg generation and function is limited to secondary lymphoid tissue or whether it can occur within the tissue-specific local environment of the cognate Ag remains
Reciprocal regulation of pH 6 antigen gene loci by PhoP and RovA in Yersinia pestis biovar Microtus.
Yiquan Zhang et al.
Future microbiology, 8(2), 271-280 (2013-02-05)
To explore the transcriptional regulation of the psaEF and psaABC loci by the RovA and PhoP regulators in Yersinia pestis. Primer extension, LacZ fusion, gel mobility shift and DNase I footprinting assays were conducted in combination for this gene regulation
Javier A Menendez et al.
Cell cycle (Georgetown, Tex.), 12(4), 555-578 (2013-02-02)
Aging can be viewed as a quasi-programmed phenomenon driven by the overactivation of the nutrient-sensing mTOR gerogene. mTOR-driven aging can be triggered or accelerated by a decline or loss of responsiveness to activation of the energy-sensing protein AMPK, a critical
Global Trade Item Number
| SKU | GTIN |
|---|---|
| G8511-1VL | 04061833644065 |
| G8511-5MG | 04061833644072 |
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