G8511
β-Galactosidase from Escherichia coli
For use as a marker in SDS-PAGE
Synonym(s):
β-D-Galactoside galactohydrolase, Lactase
mol wt
~116 kDa
packaging
vial of 0.5 mg
storage temp.
2-8°C
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General description
Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)
Application
β-Galactosidase from Escherichia coli has been used as an internal standard in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). It has been used in the protein ladder mix for optimization of microfluidic western blotting assay.
Biochem/physiol Actions
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
Other Notes
One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Microfluidic Western blotting of low-molecular-mass proteins.
Gerver RE and Herr AE
Analytical Chemistry, 86, 10625-10632 (2014)
Quantitative determination of titin and nebulin in poultry meat by SDS-PAGE with an internal standard.
Tomaszewska-Gras J, et al.
Meat Science, 62, 61-66 (2002)
Scott W McPherson et al.
Journal of immunology (Baltimore, Md. : 1950), 190(10), 4971-4981 (2013-04-16)
Extrathymically derived regulatory T cells (iTregs) protect against autoimmunity to tissue-specific Ags. However, whether Ag-specific iTreg generation and function is limited to secondary lymphoid tissue or whether it can occur within the tissue-specific local environment of the cognate Ag remains
Staci Rakowiecki et al.
Development (Cambridge, England), 140(8), 1730-1739 (2013-03-15)
The morphogenetic program that shapes the three semicircular canals (SSCs) must be executed with extreme precision to satisfy their complex vestibular function. The SSCs emerge from epithelial outgrowths of the dorsal otocyst, the central regions of which fuse and resorb
Reciprocal regulation of pH 6 antigen gene loci by PhoP and RovA in Yersinia pestis biovar Microtus.
Yiquan Zhang et al.
Future microbiology, 8(2), 271-280 (2013-02-05)
To explore the transcriptional regulation of the psaEF and psaABC loci by the RovA and PhoP regulators in Yersinia pestis. Primer extension, LacZ fusion, gel mobility shift and DNase I footprinting assays were conducted in combination for this gene regulation
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