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Merck
CN

G8880

Glutaminase from Escherichia coli

Grade V, lyophilized powder, ≥50 units/mg protein

Synonym(s):

L-Glutamine amidohydrolase

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About This Item

CAS Number:
9001-47-2
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
3.5.1.2 (BRENDA, IUBMB)
MDL number:
MFCD00131193

Key Documents

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Product Name

Glutaminase from Escherichia coli, Grade V, lyophilized powder, ≥50 units/mg protein

biological source

Escherichia coli

type

Grade V

form

lyophilized powder

mol wt

110 kDa

composition

Protein, ~30% biuret

Quality Level

200

specific activity

≥50 units/mg protein

solubility

water: 1 mg/mL (Clear w/Particle to Sl. Hazy w/Particles)

cation traces

NH4+: <0.1 μg/unit

foreign activity

NADH Oxidase ≤0.01%

storage temp.

−20°C

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Other Notes

One unit will deaminate 1.0 μmole of L-glutamine per min at pH 4.9 at 37 °C.

Physical form

Lyophilized powder containing potassium succinate and EDTA

Application

Glutaminase from E.coli has been used:
  • for the determination of glutamine from the cerebral cortex tissue extract
  • for immobilization on aminopropylsilylated controlled pore glass (CPG) for determination of glutamine
  • in combination with acivicin in in vitro melanoma cells and MCF-7 and OAW-42 cells for cell proliferation and invasiveness assay.

Biochem/physiol Actions

Glutaminase catalyzes the conversion of glutamine to glutamate.
Glutaminase from Escherichia coli along with acivicin, a glutamine analog regulates proliferation and invasiveness of cancer cells. Bacterial glutaminase is implicated in increasing the life span of cancer patients by lowering the tumor burden.

General description

Glutaminase is an amidohydrolase. It has the catalytic domain in the N-terminal and the C-terminal extension possesses STAS domain.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
0000481128
0000481127
0000481127
0000481128
0000451676

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Porta-caval shunting changes neuronal sensitivity to ammonia
Raabe W and Onstad G
Journal of the Neurological Sciences, 71(2-3), 307-314 (1985)
Modulation of metastatic potential of B16F10 melanoma cells by acivicin: synergistic action of glutaminase and potentiation of cisplatin cytotoxicity
Roy S and Maity P
Asian Pacific Journal of Cancer Prevention, 8(2), 301-301 (2007)
Functional and structural characterization of four glutaminases from Escherichia coli and Bacillus subtilis
Brown G, et al.
Biochemistry, 47(21), 5724-5735 (2008)
Ayşegül Erdem et al.
Nature communications, 13(1), 2013-2013 (2022-04-21)
Metabolic programs can differ substantially across genetically distinct subtypes of acute myeloid leukemia (AML). These programs are not static entities but can change swiftly as a consequence of extracellular changes or in response to pathway-inhibiting drugs. Here, we uncover that
Selective flow injection procedures for the determination of nitrogen containing analytes by gasdialytic-fluorimetric detection of enzymatically generated ammonia
Mana H and Spohn U
Analytica Chimica Acta, 325(1-2), 93-104 (1996)
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