G9670
Monoclonal Anti-O-Glycosylated Neurofilament-M antibody produced in mouse
~2 mg/mL, clone NL6, purified immunoglobulin, buffered aqueous solution
Synonym(s):
Anti-O-glycosylated NF-M
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
NL6, monoclonal
form
buffered aqueous solution
mol wt
antigen ~160 kDa
species reactivity
human, rat
should not react with
bovine, mouse
packaging
antibody small pack of 25 μL
concentration
~2 mg/mL
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: 0.5-1 μg/mL using human NT2 cell extracts
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... NEFM(4741)
rat ... Nefm(24588)
General description
Intermediate filaments (IFs) have a diameter of 8-10nm and differ from microtubules and microfilaments significantly. Neurofilaments (NFs) are a type of IFs found mainly in cells or tissues of neuronal origin. NFs are composed of three major proteins, NF-L, NF-M and NF-H, having a molar mass of 68kDa, 160kDa, and 200kDa, respectively.
Monoclonal Anti-O-Glycosylated Neurofilament-M (mouse IgG2a isotype) is derived from the hybridoma NL6 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a protein fraction enriched for components of the human neuronal cytoskeleton. Neurofilament medium (NEFM) gene encodes neurofilament medium polypeptide which constitutes to cytoskeleton of mature neurons. The gene in human chromosome is localized on 8p21.
Immunogen
protein fraction enriched for components of the human neuronal cytoskeleton from cell lines NT2-N and SK-N-BE(2). The epitope is localized in the carboxy-terminal tail domain of NF-M.
Application
Monoclonal Anti-O-Glycosylated Neurofilament-M antibody produced in mouse has been used in:
- immunohistochemical methods
- immunoblotting
- immunocytochemistry
Biochem/physiol Actions
Neurofilament medium (NEFM) is essential for radial growth of axon which includes the diameter of the axon as well as the myelination. The C-terminus of NFEM gene has 7 repeats of KSP (lysine-serine-proline) domain and phosphorylation of which is crucial for regulating the radial growth of axon.
The NF proteins are synthesized in the neuronal perikarya before assemby to form the filaments. These filaments are then transported within the axons towards the synaptic terminals. The function of these NF polypeptides is regulated by phosphorylation, which influences their interaction with cytoplasmic organelles. The head region of NF-L and NF-M contain sites modified by O-linked GlcNAc. Down-regulation of NF-M O-GlcNAcylation and increased phosphorylation of NF-M is observed in Alzheimer disease. This antibody recognizes specifically an O-glycosylated epitope in the projection domain of NF-M. In a rat model for amyotrophic lateral sclerosis (ALS), this specific modification is strongly decreased compared with wild-type animals.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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J A García-Sevilla et al.
Neuroreport, 8(7), 1561-1565 (1997-05-06)
NEUROFILAMENT (NF) proteins, the major components of the neuronal cytoskeleton, have been shown to represent previously unknown targets for the chronic effects of morphine in rats. This study was designed to evaluate the abundance of immunoreactive NF-L (68 kDa) proteins
Intermediate filament protein accumulation in motor neurons derived from giant axonal neuropathy iPSCs rescued by restoration of gigaxonin
Johnson KBL, et al.
Human Molecular Genetics, 24(5), 1420-1431 (2014)
Phosphorylation of neuronal cytoskeletal proteins in Alzheimer's disease and Lewy body dementias.
J Q Trojanowski et al.
Annals of the New York Academy of Sciences, 747, 92-109 (1994-12-15)
Yanqiu Deng et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 22(1), 138-145 (2007-08-10)
The medium subunit of neurofilament (NF-M) is extensively modified by phosphate and O-linked beta-N-acetylglucosamine (O-GlcNAc). Phosphorylation of NF-M plays a critical role in regulating its translocation, filament formation, and function. However, the regulation of NF-M phosphorylation and the role of
NF-M is an essential target for the myelin-directed ?outside-in? signaling cascade that mediates radial axonal growth
Garcia ML, et al.
The Journal of Cell Biology, 163(5), 1011-1020 (2003)
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