form
resin
crosslinking
cross-linked
feature
autoclavable
packaging
pack of 1 L
manufacturer/tradename
Cytiva 17-0150-01
storage condition
(20% Ethanol )
parameter
26 cm/hr max. flow rate
technique(s)
liquid chromatography (LC): suitable
matrix
4% cross-linked agarose
matrix active group
agarose
particle size
45-165 μm
pore size
60-20000 kDa fractionation range (globular proteins)
cleaning in place
3-14
operating pH
3-13
separation technique
size exclusion (SEC)
storage temp.
2-30°C
Related Categories
General description
Sepharose™ CL-4B is a well-proven cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.
Sepharose™ is a bead-formed agarose-based gel filtration matrix. Sepharose™ is available with 3 different agarose contents; 2, 4, and 6%, designated Sepharose™ 2B, Sepharose™ 4B and Sepharose™ 6B respectively, Both Sepharose™ and Sepharose™ CL have broad fractionation ranges which makes them suitable for characterizing or cleaning-up samples containing components of diverse molecular weight.
Sepharose™ CL gels are cross-linked derivatives of Sepharose™ 2B, Sepharose™ 4B and Sepharose™ 6B. The cross-linked form of Sepharose™ is chemically and physically more resistant than Sepharose™ itself, offering the same selectivity with better flow characteristics. Cross-linked Sepharose™ gels are resistant to organic solvents and are thus the choice for separations in organic solvents.
Sepharose™ is a bead-formed agarose-based gel filtration matrix. Sepharose™ is available with 3 different agarose contents; 2, 4, and 6%, designated Sepharose™ 2B, Sepharose™ 4B and Sepharose™ 6B respectively, Both Sepharose™ and Sepharose™ CL have broad fractionation ranges which makes them suitable for characterizing or cleaning-up samples containing components of diverse molecular weight.
Sepharose™ CL gels are cross-linked derivatives of Sepharose™ 2B, Sepharose™ 4B and Sepharose™ 6B. The cross-linked form of Sepharose™ is chemically and physically more resistant than Sepharose™ itself, offering the same selectivity with better flow characteristics. Cross-linked Sepharose™ gels are resistant to organic solvents and are thus the choice for separations in organic solvents.
Features and Benefits
- 4% cross-linked agarose gel filtration media.
- Proven base matrix for coupling affinity ligands
- Higher degree of crosslinking improve matrix stability
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Sepharose is a trademark of Cytiva
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
Regulatory Information
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Protocols
Selectivity of HIC medium from Cytiva is based on properties such as hydrophobicity, porosity and particle size.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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