GE17-0300-10
Ficoll® PM 400
solid, spray-dried, solubility:, soluble, non-sterile (endotoxins < 0.12 EU/mL), pack of 100 g
Synonym(s):
17-0300-10, Density gradient medium, cell culture media, cell purification media, cell storage media, lymphocyte separation medium, mononuclear cell isolation
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About This Item
sterility
non-sterile (endotoxins < 0.12 EU/mL)
form
solid
spray-dried
shelf life
5 yr
packaging
pack of 100 g
manufacturer/tradename
Cytiva 17-0300-10
dilution
(Used to form density gradients for centrifugation seperation applications)
color
White
solubility
soluble
density
≤1.2 g/mL
storage temp.
2-30°C
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General description
Features and Benefits
- For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity.
- Neutral, highly branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution.
- Concentrations of up to 50% (w/v) covering densities of up to 1.2 g/mL can be achieved.
- Better osmotic properties than sucrose.
- Preserves functional and morphological integrity.
- Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size (under conditions of sedimentation at unit gravity).
- Does not penetrate biological membranes.
- Serves as the raw material for preparation of Ficoll-Paque gradients.
- Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier.
Preparation Note
Analysis Note
Legal Information
Storage Class Code
13 - Non Combustible Solids
Regulatory Information
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Articles
The effects of the mononuclear cell separation method described by Bøyum procedure are noted below, since research situations may arise in which they are significant.
Density Marker Beads facilitate Percoll gradient monitoring in biological separations.
This page shows methods to remove Percoll from a sample after centrifugation.
Protocols
To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.
The following procedure has been evaluated with Ficoll-Paque PLUS and is recommended for separation of normal blood samples for maximum reproducibility.
为了配置Percoll梯度液，必须先用生理盐水或细胞培养基将Percoll调至生理性渗透压。
以下操作步骤已接受Ficoll-Paque PLUS评估，推荐用于分离正常血样以尽可能提高可重现性。
Related Content
Data was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll for a particular cell or tissue type.
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