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About This Item
NACRES:
NA.52
UNSPSC Code:
12352200
packaging
pkg of 750 mL
manufacturer/tradename
Cytiva 17-0476-01
matrix
spherical allyl dextran and N, N'-methylenebisacrylamide
avg. part. size
50 μm
working range
2-11
General description
Sephacryl® S-1000 gel filtration media for preparative purification of large biomolecules including DNA and large polysaccharides, proteoglycans and small particles (e.g., membrane-bound vesicles and viruses).
Sephacryl® S-1000 Superfine gel filtration medium has a high exclusion limit. The inert and highly stable matrix, specially treated to provide high recoveries of biopolymers, is prepared by covalently cross-linking allyl dextran with N,N′-methylenebisacrylamide. The high mechanical strength of the resulting beads allows high flow rates and fast separations to be achieved.
Sephacryl® S-1000 Superfine gel filtration medium has a high exclusion limit. The inert and highly stable matrix, specially treated to provide high recoveries of biopolymers, is prepared by covalently cross-linking allyl dextran with N,N′-methylenebisacrylamide. The high mechanical strength of the resulting beads allows high flow rates and fast separations to be achieved.
Features and Benefits
- For the purification of DNA up to 20 000 base pairs, dextrans up to 108 molecular weight, and spherical particles up to 400 nm.
- Yields highly pure plasmid DNA with no need for ultracentrifugation.
- Heat stability allows sterilization by autoclaving.
- Exceptional flow properties allow columns to be packed and equilibrated quickly and easily.
- Can be used in aqueous buffer systems (pH 2 to 11), in concentrated urea or in guanidine hydrochloride, as well as in a number of organic solvents.
Preparation Note
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Sephacryl is a registered trademark of Cytiva
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