Skip to Content
Merck
CN

Key Documents

View All Documentation

GE17-0510-10

Q Sepharose™ Fast Flow

Cytiva 17-0510-10, pack of 25 mL

Synonym(s):

Q Sepharose Fast Flow

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

NACRES:
NA.56
UNSPSC Code:
41106500
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

form

resin

crosslinking

cross-linked

packaging

pack of 25 mL

manufacturer/tradename

Cytiva 17-0510-10

storage condition

(20% ethanol)

technique(s)

liquid chromatography (LC): suitable

matrix

6% cross-linked agarose

matrix active group

-N+ (CH3 ) 3

particle size

45-165 μm

average diameter

90 μm

cleaning in place

2-14

working range

2-12

capacity

0.18-0.24 mmol Cl-/mL ion exchange capacity, ~42 mg binding capacity (BSA / mL resin)

separation technique

strong anion exchange

shipped in

ambient

storage temp.

2-30°C

General description

Q Sepharose™ Fast FLow is a strong anion exchanger based on the well established Sepharose™ Fast FLow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

Application

  • Well-proven strong anion exchanger developed for industrial downstream processes.
  • Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals
  • The industry standard for ion exchange chromatography during recent decades
  • High chemical stability allows for well proven CIP and sanitization protocols
  • The hydrophilic nature of the base matrix ensures Low levels of non-specific binding leading to Low levels of host cell-derived impurities in the elution pool.

Features and Benefits

Q Sepharose™ Fast FLow is part of the Sepharose™ Fast FLow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. It is composed of crosslinked 6% Agarose beads, with quaternary ammonium (Q) strong anion exchange groups. Q Sepharose™ Fast FLow has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. Scale-up with Q Sepharose™ Fast FLow is straight forward and the medium is available in a range of pre-packed process development tools.

As member of the BioProcess media range, Q Sepharose™ Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Still not finding the right product?

Explore all of our products under Q Sepharose™ Fast Flow

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Regulatory Information

新产品

This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Protocols

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

Sepharose Fast Flow: Purification with Good Resolution and Easy Scale-Up

This page covers the use of Sepharose Fast Flow for purification of proteins.

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

View All Protocols

Articles

Hydrophobic Interaction Chromatography Setup

Column, media, and sample preparation for Hydrophobic Interaction Chromatography (HIC) detailed with Cytiva media.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

View All Articles
Pär Comstedt et al.
PloS one, 9(11), e113294-e113294 (2014-11-20)
There is currently no Lyme borreliosis vaccine available for humans, although it has been shown that the disease can be prevented by immunization with an OspA-based vaccine (LYMErix). Outer surface protein A (OspA) is one of the dominant antigens expressed

Global Trade Item Number

SKUGTIN
GE17-0510-1004061837620355