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Merck
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GE17-0618-01

Protein G Sepharose™ 4 Fast Flow

Cytiva 17-0618-01, pack of 5 mL

Synonym(s):

Fast Flow resin, Antibody purification resin, IgG purification resin

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About This Item

NACRES:
NA.56
UNSPSC Code:
41106500

Key Documents

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Technical Service
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Product Name

Protein G Sepharose™ 4 Fast Flow, Cytiva 17-0618-01, pack of 5 mL

form

resin

crosslinking

cross-linked

packaging

pack of 5 mL

manufacturer/tradename

Cytiva 17-0618-01

storage condition

(20% Ehtanol)

parameter

<0.1 Mpa pressure
150-250 cm/hr flow rate

technique(s)

liquid chromatography (LC): suitable

matrix

4% cross-linked agarose

matrix active group

Recombinant streptococcal protein G lacking the albumin-binding region, produced in E. coli

average diameter

90 μm

cleaning in place

2-10

working range

3-9

capacity

≥20 mg binding capacity(human IgG/mL resin)

separation technique

affinity

storage temp.

2-8°C

Related Categories

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Features and Benefits

  • Binding specificities that complement Protein A media.
  • Binds a broad range of IgG species and subclasses.
  • Multi-point attachment minimizes ligand leakage.
  • Used in a range of research applications.

General description

Protein G Sepharose™ 4 Fast Flow is recombinant protein G coupled to Sepharose™ 4 Fast Flow.

Protein G Sepharose™ 4 Fast Flow has recombinant protein G immobilized by the cyanogen bromide (CNBr) method to Sepharose™ 4 Fast Flow. Protein G exhibit binding specificities that complement Protein A media and binds to the Fc region of IgG from a variety of mammalian species. Protein G Sepharose™ 4 Fast Flow may be used to isolate and purify classes, subclasses and fragments of immunoglobulins from any biological fluid or cell culture medium.

As member of the BioProcess media range, Protein G Sepharose™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Legal Information

Sepharose is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Regulatory Information

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Yunhao Tan et al.
Methods in molecular biology (Clifton, N.J.), 1714, 79-95 (2017-11-28)
Ligand-induced macromolecular protein complex formation has emerged as a common means by which the innate immune system activates signal transduction pathways essential for host defense. Despite their structural divergence, key signaling molecules in diverse innate immune pathways mediate signal transduction
Ting-Ting Du et al.
Nature communications, 10(1), 1117-1117 (2019-03-10)
Sensory hair cells, the mechanoreceptors of the auditory and vestibular systems, harbor two specialized elaborations of the apical surface, the hair bundle and the cuticular plate. In contrast to the extensively studied mechanosensory hair bundle, the cuticular plate is not
Marti Quevedo et al.
Nature communications, 10(1), 2669-2669 (2019-06-19)
The Mediator complex regulates transcription by connecting enhancers to promoters. High Mediator binding density defines super enhancers, which regulate cell-identity genes and oncogenes. Protein interactions of Mediator may explain its role in these processes but have not been identified comprehensively.
Bodan Hu et al.
Bio-protocol, 10(4), e3523-e3523 (2021-03-04)
Non-covalent binding of cholesterol to the transmembrane region of proteins affect their functionalities, but methods to prove such an interaction are rare. We describe our protocol to label the hemagglutinin (HA) of Influenza virus with a cholesterol derivative in living
Nezha S Benabdallah et al.
Molecular cell, 76(3), 473-484 (2019-09-09)
Enhancers can regulate the promoters of their target genes over very large genomic distances. It is widely assumed that mechanisms of enhancer action involve the reorganization of three-dimensional chromatin architecture, but this is poorly understood. The predominant model involves physical
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Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Immunoprecipitation Techniques

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

Protein G and Protein A Bind to Different IgG

This page shows a comparison of the relative binding strengths of protein G and protein A to different immunoglobulins.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

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Protocols

Performing a Purification of IgG Antibodies with Protein G Sepharose® 4 Fast Flow

This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

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