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Merck
CN

GE17-0700-01

2′,5′ ADP-Sepharose™ 4B

Cytiva 17-0700-01, pack of 5 g

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

Key Documents

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Technical Service
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packaging

pack of 5 g

manufacturer/tradename

Cytiva 17-0700-01

matrix

4% agarose

particle size

45-165 μm

average diameter

90 μm

cleaning in place

4-10

working range

4-10

shipped in

wet ice

storage temp.

2-8°C

General description

2′5′ ADP Sepharose™ 4B media with immobilized NADP structural analog is primarily used for purification of enzymes requiring NADP as a cofactor.

Application

2′5′ ADP Sepharose™ 4B interacts strongly with NADP+-dependent dehydrogenases. Selective elution with gradients of NAD+ or NADP+ has allowed the resolution of complex mixtures of dehydrogenase isoenzymes using 2′5′ ADP Sepharose™ 4B.

Features and Benefits

  • 2′5′ ADP is an NADP structural analog immobilized on Sepharose™ 4B. It binds and immobilizes enzymes requiring this cofactor

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Storage Class Code

13 - Non Combustible Solids

Regulatory Information

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Performing a Purification of NADP+-dependent dehydrogenases and other enzymes with affinity for NADP+ Using 2’5’ ADP Sepharose 4B and Red Sepharose CL-6B

This page shows how to purify NADP+-dependent dehydrogenases and other enzymes with affinity for NADP+ by affinity chromatography using 2’5’ ADP Sepharose 4B and Red Sepharose CL-6B from Cytiva.

Protocols

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

Column Packing and Preparation Protocol for Affinity Chromatography

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.

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该页面展示了如何使用Cytiva的Tricorn™或XK色谱柱进行亲和层析色谱柱填充和制备。

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