form
prepacked column
crosslinking
cross-linked
mol wt
protein Mr 10 MDa (exclusion limit)
packaging
pack of 5 × 1 mL
manufacturer/tradename
Cytiva 17-1153-01
storage condition
(20% ethanol)
parameter
1 mL/min flow rate (recommended), 4 mL/min max. flow rate, 5 bar max. pressure
technique(s)
liquid chromatography (LC): suitable
bed volume
1 mL
column I.D. × L
7 mm × 25 mm
matrix
cross-linked agarose, spherical
matrix active group
-CH2 N+ (CH3 ) 3, quaternary ammonium
particle size
24-44 μm
avg. part. size
34 μm
cleaning
2-14
working range
2-12
capacity
0.14-0.2 mmol Cl-/mL ion exchange capacity, ~70 mg binding capacity (BSA / mL resin)
separation technique
anion exchange
shipped in
ambient
storage temp.
2-30°C
General description
HiTrap® Q HP are prepacked, ready-to-use Q Sepharose™ High Performance strong anion exchange columns for high-resolution, small-scale protein purification.
Application
Techniques & Applications: Separation of protein using a strong anion exchange medium prepacked in the HiTrap® column format.
Features and Benefits
- 34 μm bead size for high-performance, high-resolution purifications.
- Convenient and reproducible for fast, easy, high-performance separations either alone or connected in series.
- Designed for use with a syringe, peristaltic pump, and chromatography systems such as AKTA design.
- Strong quaternary ammonium (Q) anion exchanger
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Other Notes
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Legal Information
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
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signalword
Warning
hcodes
存储类别
3 - Flammable liquids