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Merck
CN

GE17-1440-03

Ficoll® Paque Plus

liquid, sterile (endotoxins < 0.12 EU/mL), density: 1.077  g/mL, pack of 6 × 500 mL

Synonym(s):

Ficoll density gradient medium, cell culture media

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About This Item

UNSPSC Code:
41105330
NACRES:
NA.25
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sterility

sterile (endotoxins < 0.12 EU/mL)

form

liquid

shelf life

3 yr

packaging

pack of 6 × 500 mL

manufacturer/tradename

Cytiva 17-1440-03

storage condition

protect from light

dilution

(purification of mononuclear cells from human peripheral blood)

color

Colorless to slightly yellow

pH range

5.5-7.5

density

1.077  g/mL

General description

Ficoll-Paque Plus is a recognized standard in laboratories worldwide for the isolation of human lymphocytes for in vitro studies.

Ficoll-Paque PLUS is a ready to use, sterile medium for isolation of lymphocytes in high yield from peripheral blood using a simple and rapid centrifugation procedure. It maintains the viability and a representative distribution of B and T lymphocytes.

Application

For in vitro isolation of lymphocytes from human peripheral blood.

Features and Benefits

  • Ready to use sterile medium for isolation of human lymphocytes in high yield from peripheral blood.
  • Maintains viability and representative distribution of B and T lymphocytes.
  • Low levels of endotoxin (< 0.12 EU/mL).

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Ficoll is a registered trademark of Cytiva

Storage Class Code

12 - Non Combustible Liquids

Regulatory Information

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Kamalesh Dattaram Mumbrekar et al.
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Pseudomonas aeruginosa biofilms represent a major threat to health care. Rugose small colony variants (RSCV) of P. aeruginosa, isolated from chronic infections, display hyperbiofilm phenotype. RSCV biofilms are highly resistant to antibiotics and host defenses. This work shows that RSCV
Chelsea E Stamm et al.
mSphere, 4(3) (2019-06-07)
Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis, is one of the most successful human pathogens. One reason for its success is that Mtb can reside within host macrophages, a cell type that normally functions to phagocytose and destroy infectious

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