GE17-3712-02
Ni Sepharose™ Excel
Cytiva 17-3712-02, pack of 100 mL
Synonym(s):
Ni Sepharose Excel
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About This Item
form
resin
crosslinking
cross-linked
packaging
pack of 100 mL
manufacturer/tradename
Cytiva 17-3712-02
storage condition
(20% ethanol)
parameter
600 cm/hr max. flow rate
technique(s)
liquid chromatography (LC): suitable
matrix
Highly cross-linked spherical agarose
matrix active group
Nickel
particle size
~90 μm
cleaning in place
2-14
operating pH
2-12
capacity
≥10 mg binding capacity (histidine-tagged protein)
separation technique
affinity
storage temp.
2-30°C
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General description
EDTA-resistant resin for IMAC chromatography: Ni Sepharose excel is an IMAC resin precharged with nickel ions that are very strongly bound to a chelating ligand. Nickel ions remain bound to the ligand even after 24 h incubation in 10 mM EDTA. Because of this strong binding, samples that typically cause stripping of metal ions can be loaded directly onto the resin without time-consuming pretreatment. Therefore, this his tag purification resin is especially suitable for purifying histidine-tagged (his-tagged) proteins secreted into eukaryotic cell culture supernatants.
His tag purification of large sample volumes: The flow properties of Ni Sepharose excel nickel resin make it well-suited to purification at a wide range of scales. This resin can handle loading of large sample volumes, so you can purify low concentrations of target proteins using manual or automated methods.
Convenient prepacked columns:
- HisTrap excel column: HiTrap format for convenient use with a syringe, a peristaltic pump, or a chromatography system.
- His MagSepharose excel: Magnetic beads for small-scale purification and screening. They are appropriate for early stages of research when many parallel experiments are required.
Features and Benefits
- Versatile – suitable for eukaryotic samples, including insect and CHO cells, as well as bacterial samples such as E. coli.
- Direct sample load – of eukaryotic cell culture supernatants, eliminating the need for pretreatment of samples that usually cause nickel stripping.
- Retained binding capacity of up to 10 mg/mL resin.
- Simplified sample handling – fewer steps needed compared with conventional workflow.
- Flexible formats – prepacked and loose resin formats for optimized screening and preparative his-tag purification.
Preparation Note
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Regulatory Information
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Articles
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
How to optimize purification of histidine-tagged proteins using Cytiva products.
This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.
Protocols
This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using Ni Sepharose Excel from Cytiva.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
This protocol shows how to remove histidine tags by enzymatic cleavage using Cytiva products.
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