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GE28-4013-51

His GraviTrap™ Kit

Cytiva 28-4013-51, kit of one pack of His buffer, pack of 2 × 10 columns

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About This Item

NACRES:
NA.25
UNSPSC Code:
12352200

Key Documents

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Technical Service
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manufacturer/tradename

Cytiva 28-4013-51

packaging

kit of one pack of His buffer, pack of 2 × 10 columns

storage condition

(20% Ethanol)

bed volume

1 mL

capacity

40 mg binding capacity (histidine-tagged protein/column)(Protein binding capacity is protein-to-protein dependent.)

storage temp.

2-8°C

General description

His Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of histidine-tagged proteins according to recommended protocols. Moreover, method for optimization of imidazole concentration in the binding buffer is described.

His GraviTrap is a prepacked, single-use column for purification of histidine-tagged proteins by immobilized metal affinity chromatography (IMAC). Column gives fast and simple gravity-flow purifications without any need for a purification.

The ready to use column is prepacked with Ni Sepharose 6 Fast Flow, which has a high protein binding capacity, low nickel-ion leakage, and is compatible with denaturing and reducing agents, as well a wide range of additives.

His GraviTrap columns are delivered in a package that can be converted into a column stand to simplify purification. LabMate PD-10 Buffer Reservoir can be connected to the columns for convenient handling of sample volumes above 10 mL.
The Buffer kit can also be separately purchased:.
The His GraviTrap can also be separately purchased:.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

GraviTrap is a trademark of Cytiva

pictograms

Health hazard
GHS08

signalword

Danger

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Regulatory Information

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Protocols

Ni Sepharose 6 Fast Flow for Protein Purification

Ni Sepharose 6 Fast Flow purifies histidine-tagged proteins efficiently, offering high cross-linked agarose beads with Ni2+ ions.

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

Gravity-flow Purification using His GraviTrap™ and His GraviTrap™ Kit

This page shows how to purify histidine-tagged recombinant proteins by gravity flow using His GraviTrap and His GraviTrap Kit from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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