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Merck
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GE28-9031-30

Streptavidin High Performance Spintrap™

Cytiva 28-9031-30, pack of 16 columns

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About This Item

NACRES:
NA.25
UNSPSC Code:
41106500

Key Documents

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Technical Service
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feature

wetted part: no

packaging

pack of 16 columns

manufacturer/tradename

Cytiva 28-9031-30

storage condition

(20% Ehtanol), (20% Ethanol)

bed volume

800 μL

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

cleaning

2-10.5

working range

4-9

capacity

>300 nmol/mL binding capacity(biotin/ml medium 6 mg biotinylated BSA/ml medium), binding capacity(>300 nmol biotin/ml medium 6 mg biotinylated BSA/ml medium)

storage temp.

2-8°C

General description

Streptavidin HP SpinTrap™ are prepacked, single-use spin columns and 96-well filter plates for the preparation of protein samples and enrichment of proteins of interest from clarified cell lysates and biological fluids.

Application

Streptavidin HP SpinTrap™ can be used for Protein enrichment, where a biotinylated antibody (or similar affinity molecule) is attached to the streptavidin and the Protein of interest is enriched through the affinity interaction with the antibody. The columns can also be used for the direct enrichment of Proteins that are biotinylated. By binding a specific biotinylated Protein to the column, Protein-Protein interactions can also be investigated.

Features and Benefits

  • For simple, small-scale Protein enrichment through immobilized biotinylated biomolecules.
  • Fast and flexible protocol with elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
  • Useful for exploiting either the strong interaction of biotin and streptavidin or the somewhat weaker interaction of 2-iminobiotin and streptavidin.
  • Each column is packed with Streptavidin Sepharose™ High Performance for reproducibility and high performance.
  • Easy scale-up with HiTrap® Streptavidin HP prepacked columns.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Regulatory Information

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Certificates of Analysis (COA)

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Protocols

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Purification or Removal of Biotin and Biotinylated Substances

This page shows how to purify or remove biotin and biotinylated substances with Streptavidin Sepharose High Performance from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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