GE28-9909-44
Superdex® 200 Increase 10/300 GL
Cytiva 28-9909-44, column L × I.D. 30 cm × 10 mm, 8.6 μm particle size
Synonym(s):
Superdex Increase
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About This Item
NACRES:
SB.52
UNSPSC Code:
41115700
material
glass column, polyethylene filter
product line
Superdex®
form
prepacked column
crosslinking
cross-linked
mol wt
protein Mr 1.3 MDa (exclusion limit), protein Mr 10-600 kDa (fractionation range), Mp 1-100 kDa (dextrans fractionation range)
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 28-9909-44
storage condition
(20% ethanol)
parameter
0.75 mL/min flow rate, 1.8 mL/min max. flow rate, 25-500 μL sample volume, 3.0 Mpa pressure drop required
technique(s)
liquid chromatography (LC): suitable
bed volume
~24 mL
column L × I.D.
30 cm × 10 mm
matrix
cross-linked agarose-dextran composite
matrix active group
dextran-agarose
particle size
8.6 μm
cleaning
1-14
operating pH
3—12
separation technique
size exclusion (SEC)
shipped in
ambient
storage temp.
2-30°C
General description
Superdex® 200 Increase size exclusion chromatography columns are well suited for high-resolution analysis and small-scale purification of monoclonal antibodies (mAb) and other biomolecules with Mr~ 10 000 to ~ 600 000.
Superdex™ 200 Increase is a next-generation, dextran-agarose composite matrix for SEC. With smaller and more rigid beads than their predecessors, Superdex™ Increase columns deliver higher resolution purification in shorter run times.
Superdex™ 200 Increase has a selectivity curve optimized for excellent resolution of antibodies and macromolecules within the range Mr ~ 100 000 to ~ 300 000. It is well suited for detection and separation of antibody monomers, dimers, and aggregates present in monoclonal antibody preparations. The outstanding resolving power of this resin facilitates protein characterization, including analysis of antibody size variants, assessment of membrane protein size homogeneity, and the study of protein-protein interactions.
Superdex™ 200 Increase is optimized to give high resolution separation for proteins with molecular weights in the range Mr ~ 100 000 to ~ 300 000. Superose™ 6 Increase provides a complementary fractionation range, resolving very large protein complexes and macromolecules that Superdex 200 Increase cannot separate.
This agarose-based resin is alkali-resistant and supports cleaning-in-place (CIP) procedures. This capability allows the same column to be used for different proteins, with minimal risk of carry-over between samples.
Superdex™ 200 Increase is a next-generation, dextran-agarose composite matrix for SEC. With smaller and more rigid beads than their predecessors, Superdex™ Increase columns deliver higher resolution purification in shorter run times.
Superdex™ 200 Increase has a selectivity curve optimized for excellent resolution of antibodies and macromolecules within the range Mr ~ 100 000 to ~ 300 000. It is well suited for detection and separation of antibody monomers, dimers, and aggregates present in monoclonal antibody preparations. The outstanding resolving power of this resin facilitates protein characterization, including analysis of antibody size variants, assessment of membrane protein size homogeneity, and the study of protein-protein interactions.
Superdex™ 200 Increase is optimized to give high resolution separation for proteins with molecular weights in the range Mr ~ 100 000 to ~ 300 000. Superose™ 6 Increase provides a complementary fractionation range, resolving very large protein complexes and macromolecules that Superdex 200 Increase cannot separate.
This agarose-based resin is alkali-resistant and supports cleaning-in-place (CIP) procedures. This capability allows the same column to be used for different proteins, with minimal risk of carry-over between samples.
Application
For both preparative and analytical purposes
Gel filtration for small-scale (25-500 mL) preparative purification.
Long term use: all commonly used aqueous buffers, up to 8 M urea, ionic and non-ionic detergents, up to 6M guanidine hydrochloride
Features and Benefits
- Enhanced performance: improved resolution and runtime compared to predecessor Superdex™ 200.
- Very high resolution: small bead size and narrow particle size distribution provide high resolution, for high protein purity.
- High flow rates: rigid beads give excellent pressure/flow properties.
Preparation Note
Storage Temp.: 4°C to 30°C
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Legal Information
Superdex is a registered trademark of Cytiva
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
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