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Merck
CN

GE29-0362-32

Superdex® 200 PC 3.2/300

Cytiva 29-0362-32, column L × I.D. 30 cm × 3.2 mm, 13 μm avg. part. size

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About This Item

UNSPSC Code:
12352200

Key Documents

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material

borosilicate glass column
titanium frit

mol wt

10000-600000 Da (globular proteins)

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 29-0362-32

availability

not available in USA, Canada and Puerto Rico

parameter

<50 mL sample volume
0.01-0.1 mL/min flow rate
1.5 Mpa max. pressure (217 psi)

column L × I.D.

30 cm × 3.2 mm

avg. part. size

13 μm

working range

3-12

separation technique

size exclusion (SEC)

General description

Superdex 200 3.2/300 is a prepacked Precision Column (2.4 mL) designed for sensitive and high-resolution gel filtration separations of biomolecules in the micropreparative scale. Superdex 200 has a separation range for biomolecules with molecular weights between 10 000 and 600 000.

Application

Columns are not suitable for use with ÄKTAprime plus system.
Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol, 70% ethanol, 1 M NaOH (for cleaning in place)

Features and Benefits

  • Excellent choice for the polishing step in a purification procedure.
  • Precision columns (PC) for micropurification and analysis: sample volumes of 4 - 50 μL.
  • Easy and predictable scale-up.
  • Excellent reproducibility and durability.
  • New hardware design for convenient direct connection to high performance chromatography systems such as ÄKTA design.

Preparation Note

Store at 4 to 30 °C (20% ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Superdex is a registered trademark of Cytiva

Regulatory Information

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