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GE29-0915-96

Sigma-Aldrich

Superose® 6 Increase 10/300 GL

Cytiva 29-0915-96, 10/300 GL

Synonym(s):

Superose® 6 Increase

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

Key Documents

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material

glass column
polyethylene filter

product line

Superose®

form

prepacked column

crosslinking

cross-linked

mol wt

protein Mr 40 MDa (exclusion limit)
protein Mr 5-5000 kDa (fractionation range)
Mp 1-300 kDa (dextrans fractionation range)

packaging

package of 1 ea

manufacturer/tradename

Cytiva 29-0915-96

storage condition

(20% ethanol )

parameter

0.5 mL/min flow rate (recommended)
1.50 mL/min max. flow rate
25-500 μL sample volume
3 Mpa pressure drop required

technique(s)

liquid chromatography (LC): suitable

L × I.D.

300 mm × 10 mm

bed volume

24 mL

matrix

cross-linked agarose

matrix active group

agarose

particle size

~ 8.6 μm

cleaning

1-14

working range

3-12

separation technique

size exclusion (SEC)

shipped in

ambient

storage temp.

2-30°C

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General description

Superose® 6 Increase 10/300 GL is a versatile, prepacked column for high-resolution size exclusion chromatography in small-scale (< 500 μl sample volume) preparative purification, as well as for characterization and analysis of proteins and other biomolecules with molecular weights between 5 000 and 5 000 000.

Application

Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place)
Superose® 6 Increase 10/300 GL column is suitable for small-scale (25 – 500 μl sample volume) preparative purification for obtaining milligram amounts of highly size-homogenous target proteins at the polishing stage of a purification procedure. It is also well-suited for the characterization of large protein complexes and for the study of protein-protein interactions.

Features and Benefits

  • Increased resolution compared with Superose 6, for higher purity and improved analysis results.
  • Reduced runtime compared with Superose 6, for rapid results.
  • Versatile use for both preparative and analytical purposes, especially for large proteins and complexes.
  • Tolerance to repeated harsh cleaning procedures at high pH, giving long column lifetime and minimal carry-over.

Preparation Note

Storage Temp.: 4°C to 30°C
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Superdex is a registered trademark of Cytiva
Superose is a registered trademark of Cytiva

Storage Class Code

3 - Flammable liquids

Regulatory Information

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Anja Fux et al.
Cell chemical biology, 26(1), 48-59 (2018-11-13)
Detection of dynamic protein-protein interactions within complexes and networks remains a challenging task. Here, we show by the example of the proteolytic ClpXP complex the utility of combined chemical cross-linking and mass spectrometry (XL-MS) to map interactions within ClpP and

Articles

Setting Pressure Limits for Size Exclusion Chromatography

Understanding pressure drop in chromatography systems is crucial for optimal functionality across different parts of the system.

Protocols

Performing a Separation with Superose

Superose from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superose column.

Sample Preparation for Size Exclusion Chromatography

Ensure chromatographic sample clarity to avoid column clogging, reduce washing needs, and prolong column life for purification.

Related Content

Separation Options with Superose®

Separation options for Size Exclusion Chromatography with Superose from Cytiva.

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