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Merck
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GW22306F

Sigma-Aldrich

Anti-QPRT antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
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biological source

chicken

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

manufacturer/tradename

Genway 15-288-22306F

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... QPRT(23475)

General description

QPRT (quinolinate phosphoribosyltransferase) is an essential enzyme of quinolinate catabolism, and has a molecular weight of 34kDa. It is found in both the cytoplasm and nucleus, and is expressed in central nervous system cells, platelets, erythrocytes and liver. It belongs to the type II phosphoribosyltransferase family. Its active site is situated at an α/β open sandwich region.

Immunogen

Immunogen Sequence: GI # 45269149, sequence 123-237
Recombinant quinolinate phosphoribosyltransferase

Application

Anti-QPRT antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Biochem/physiol Actions

Mammalian quinolinate phosphoribosyltransferase (QPRTase) is a key enzyme in catabolism of quinolinate. It is an intermediate in the tryptophan-nicotinamide adenine dinucleotide (NAD) pathway. Quinolinate acts as a potent endogenous exitotoxin to neurons and an elevation in its levels in the brain is linked to the pathogenesis of neurodegenerative disorders. QPRT acts at the junction of two different pathways, de novo NAD+ biosynthesis and the kynurenine pathway of tryptophan degradation. It is an important enzyme in terms of its biological impact and its potential as a therapeutic target.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Shuja S Malik et al.
Proteins, 82(3), 405-414 (2013-09-17)
Quinolinic acid (QA), a biologically potent but neurodestructive metabolite is catabolized by quinolinic acid phosphoribosyltransferase (QPRT) in the first step of the de novo NAD(+) biosynthesis pathway. This puts QPRT at the junction of two different pathways, that is, de
Nora Hinsch et al.
BMC cancer, 9, 93-93 (2009-03-27)
The differential diagnosis between follicular thyroid adenoma and minimal invasive follicular thyroid carcinoma is often difficult for several reasons. One major aspect is the lack of typical cytological criteria in well differentiated specimens. New marker molecules, shown by poly- or
S I Fukuoka et al.
Biochimica et biophysica acta, 1395(2), 192-201 (1998-02-25)
Mammalian quinolinate phosphoribosyltransferase (QPRTase) (EC 2.4.2.19) is a key enzyme in catabolism of quinolinate, an intermediate in the tryptophan-nicotinamide adenine dinucleotide (NAD) pathway. Quinolinate acts as a most potent endogenous exitotoxin to neurons. Elevation of quinolinate levels in the brain
Huanting Liu et al.
Journal of molecular biology, 373(3), 755-763 (2007-09-18)
Human quinolinate phosphoribosyltransferase (EC 2.4.2.19) (hQPRTase) is a member of the type II phosphoribosyltransferase family involved in the catabolism of quinolinic acid (QA). It catalyses the formation of nicotinic acid mononucleotide from quinolinic acid, which involves a phosphoribosyl transfer reaction

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