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GW22535

Sigma-Aldrich

Anti-EIF353 antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

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About This Item

UNSPSC Code:
12352203
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biological source

chicken

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human, mouse, rat

manufacturer/tradename

Genway 15-288-22535

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... EIF3H(8667)

General description

EIF3H (eukaryotic translation initiation factor 3, subunit H) is a part of a ~600kDa, multi-protein complex called eIF3, which is made of ten different subunits. This subunit is also called p40. This gene is localized to human chromosome 8q23.3.

Immunogen

Immunogen Sequence: GI # 4503515, sequence 1-352
Recombinant eukaryotic translation initiation factor 3, subunit 3 gamma, 40kDa

Application

Anti-EIF353 antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Biochem/physiol Actions

EIF3H (eukaryotic translation initiation factor 3, subunit H) is a component of EIF3, which facilitates the binding of methionyl-tRNAi and mRNA, by interacting with 40S ribosome. The up-regulation of this gene leads to elevation in cell proliferation, growth and survival. This gene is amplified in breast and liver cancer. The expression of EIF3H is increased in prostate cancer. It helps in regulating cell growth and viability. Overexpression of the gene may provide growth advantage to the cancer cells.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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K Asano et al.
The Journal of biological chemistry, 272(43), 27042-27052 (1997-10-27)
The mammalian translation initiation factor 3 (eIF3), is a multiprotein complex of approximately 600 kDa that binds to the 40 S ribosome and promotes the binding of methionyl-tRNAi and mRNA. cDNAs encoding 5 of the 10 subunits, namely eIF3-p170, -p116
Federico Cappuzzo et al.
Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer, 4(4), 472-478 (2009-02-11)
We investigated the incidence of eukaryotic translation initiation factor 3 subunit H (EIF3H) and MYC amplification in non-small cell lung cancer (NSCLC) patients, and whether MYC/EIF3H increased gene copy number affected response to Epidermal Growth Factor Receptor tyrosine kinase inhibitors.
Kimmo J Savinainen et al.
The Prostate, 66(11), 1144-1150 (2006-05-03)
Amplification and overexpression of EIF3S3 gene has been demonstrated in breast and prostate cancer. Here, our goal was to study the effect of EIF3S3 on cell growth. The effect of EIF3S3 on growth of NIH 3T3 murine fibroblasts as well
K J Savinainen et al.
British journal of cancer, 90(5), 1041-1046 (2004-03-05)
The long arm of chromosome 8 is one of the most common regions of amplification in cancers of several organs, especially carcinomas of the breast and prostate. TRPS1, MYC and EIF3S3 genes are located in one of the minimal regions
Alan M Pittman et al.
PLoS genetics, 6(9), e1001126-e1001126 (2010-09-24)
Common genetic variation at human 8q23.3 is significantly associated with colorectal cancer (CRC) risk. To elucidate the basis of this association we compared the frequency of common variants at 8q23.3 in 1,964 CRC cases and 2,081 healthy controls. Reporter gene

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