H1290
HIV Protease Mutant
recombinant, expressed in E. coli
Synonym(s):
(L33F, M46L, I54V, V82A, L90M, L10I, M36I, A71V, L63P)
recombinant
expressed in E. coli
form
solution
mol wt
~10.8 kDa
shipped in
dry ice
storage temp.
−70°C
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Features and Benefits
Stabilized without non-physiologic mutations
Packaging
100 unit vial containing ≥ 100ug protein
Physical form
Supplied as a solution in 0.5M Sodium Acetate, pH 4.7with 50mM Tris-HCL, 1M NaCL, 1mM EDTA, 5mM DTT and 15% glycerol.
Preparation Note
Bioengineered to resist autoproteolysis, resulting in an enzyme with improved stability and substantially higher activity levels over extended time periods to conduct kinetic assays. The enzyme includes the most commonly occurring background polymorphism (L63P) that aids in the drug resistance profile.
Other Notes
One unit of HIV protease will cleave 1picomole per minute of the substrate Abz-Ala-Arg-Val-Nle-Tyr(NO2)-Glu-Ala-Nle-NH2 at 37°C.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
常规特殊物品
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