H2917
Hexokinase-2 human
1 mg/mL, buffered aqueous glycerol solution
Synonym(s):
DKFZp686M1669, HK II, HK2, HXK2, Hexokinase type II, Muscle form hexokinase
recombinant
expressed in E. coli
form
buffered aqueous glycerol solution
specific activity
3-4 units/mL
mol wt
104.1 kDa
concentration
1 mg/mL
relevant disease(s)
cancer
shipped in
wet ice
storage temp.
−20°C
General description
Hexokinase 2 is the predominant form found in skeletal muscle.
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Hexokinase 2 is the predominant form found in skeletal muscle.
Application
Hexokinase 2 (HK2) is a glycolytic enzyme that is crucial for the Warburg effect, which is the increased rate of glycolysis seen in rapidly growing cancer cells. HK2 may be used in cancer research to study human glioblastoma multiforme (GBM), which is the most common malignant brain tumor . HK2 may be a potential therapeutic target for breast cancer as HK2 overexpression is associated with metastasis to the brain .
Biochem/physiol Actions
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Hexokinase 2 is involved in glucose metabolism and apoptosis .
Physical form
Supplied in a solution containing 20 mM Tris pH 8.0 and 10% glycerol.
Other Notes
One unit will produce 1.0 μmole NADPH per minute as glucose is phosphorylated by ATP at pH 7.4 at 30 deg C.
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Diane Palmieri et al.
Molecular cancer research : MCR, 7(9), 1438-1445 (2009-09-03)
Brain metastases of breast cancer seem to be increasingin incidence as systemic therapy improves. Metastatic disease in the brain is associated with high morbidity and mortality. We present the first gene expression analysis of laser-captured epithelial cells from resected human
Amparo Wolf et al.
The Journal of experimental medicine, 208(2), 313-326 (2011-01-19)
Proliferating embryonic and cancer cells preferentially use aerobic glycolysis to support growth, a metabolic alteration commonly referred to as the "Warburg effect." Here, we show that the glycolytic enzyme hexokinase 2 (HK2) is crucial for the Warburg effect in human
Structural biology: Security measures of a master regulator.
Dario R Alessi et al.
Nature, 497(7448), 193-194 (2013-05-03)
Helen S Bateup et al.
Neuron, 78(3), 510-522 (2013-05-15)
Neural circuits are regulated by activity-dependent feedback systems that tightly control network excitability and which are thought to be crucial for proper brain development. Defects in the ability to establish and maintain network homeostasis may be central to the pathogenesis
Venus Thomanetz et al.
The Journal of cell biology, 201(2), 293-308 (2013-04-10)
The mammalian target of rapamycin (mTOR) assembles into two distinct multi-protein complexes called mTORC1 and mTORC2. Whereas mTORC1 is known to regulate cell and organismal growth, the role of mTORC2 is less understood. We describe two mouse lines that are
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