H5163
Hedgehog-interacting Protein from mouse
lyophilized powder, ≥90% (SDS-PAGE), recombinant, expressed in NSO cells
Synonym(s):
Hip
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About This Item
MDL number:
UNSPSC Code:
51111800
biological source
mouse
recombinant
expressed in NSO cells
Assay
≥90% (SDS-PAGE)
form
lyophilized powder
storage condition
avoid repeated freeze/thaw cycles
impurities
endotoxin, tested
UniProt accession no.
storage temp.
−20°C
Gene Information
mouse ... Hhip(15245)
Biochem/physiol Actions
Hhip (hedgehog-interacting protein) from mouse is a membrane-associated glycoprotein and an antagonist of hedgehog (Hh) signalling. Mice embryos lacking HIP1 have lung defects and die after birth. Hip interacts with mammalian hedgehog family proteins, namely Shh (sonic hedgehog), Ihh (indian hedgehog) and Dhh (desert hedgehog). It is transcriptionally regulated by Hh signalling. Hh proteins are crucial for controlling cell proliferation and cell fate.
Features and Benefits
Based on N-terminal sequencing, this preparation contains a mixture of at least two peptides that have Lys 24 and Asp 52, respectively, at the N-terminus. The two peptides have calculated molecular masses of ~76 kDa and 73 kDa, respectively. Due to glycosylation and C-terminal proteolytic processing, recombinant mouse Hip migrates with apparent molecular masses of 80-90 kDa and 58-64 kDa, respectively, in SDS-PAGE under reducing conditions.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.
Analysis Note
The biological activity is measured by its ability to inhibit sonic hedgehog (Shh) induction of alkaline phosphatase production in C3H10T1/2 fibroblasts.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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P T Chuang et al.
Nature, 397(6720), 617-621 (1999-03-02)
The Hedgehog signalling pathway is essential for the development of diverse tissues during embryogenesis. Signalling is activated by binding of Hedgehog protein to the multipass membrane protein Patched (Ptc). We have now identified a novel component in the vertebrate signalling
M Bak et al.
Cytogenetics and cell genetics, 92(3-4), 300-303 (2001-07-04)
Hedgehog-interacting protein (Hhip) is a novel regulatory component in the vertebrate hedgehog-signalling pathway. The murine Hhip encodes a type I TM protein that attenuates hedgehog signalling by binding all three mammalian hedgehog proteins. Here we describe the cloning and characterisation
Juhee Jeong et al.
Development (Cambridge, England), 132(1), 143-154 (2004-12-04)
Upregulation of Patched (Ptc), the Drosophila Hedgehog (Hh) receptor in response to Hh signaling limits the range of signaling within a target field by sequestering Hh. In vertebrates, Ptch1 also exhibits ligand-dependent transcriptional activation, but mutants lacking this response show
Catherine L Olsen et al.
BMC cancer, 4, 43-43 (2004-08-06)
The Hedgehog (Hh) signaling pathway regulates a variety of developmental processes, including vasculogenesis, and can also induce the expression of pro-angiogenic factors in fibroblasts postnatally. Misregulation of the Hh pathway has been implicated in a variety of different types of
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