H6024
Hoechst Stain solution
Synonym(s):
Bisbenzimide H 33258
InChI
1S/C27H28N6O.3ClH.H2O/c1-32-10-12-33(13-11-32)22-5-9-25-21(15-22)17-29-27(31-25)19-4-8-24-20(14-19)16-28-26(30-24)18-2-6-23(34)7-3-18;;;;/h2-9,14-15,34H,10-13,16-17H2,1H3,(H,28,30)(H,29,31);3*1H;1H2
SMILES string
O.Cl.Cl.Cl.CN1CCN(CC1)c2ccc3NC(=NCc3c2)c4ccc5NC(=NCc5c4)c6ccc(O)cc6
InChI key
OWRSPPSBNWJJAR-UHFFFAOYSA-N
form
solution
application(s)
histology
storage temp.
2-8°C
Application
A fluorescent DNA stain qualified for use in Mycoplama staining.
General description
Hoechst stains are part of a family of blue fluorescent dyes commonly used to stain DNA. It gets incorporated biosynthetically into interphase nuclei in fixed cytologic preparations and in unfixed cultured cells. It can also be used to detect biosynthetic incorporation of 5-bromodeoxyuridine into the DNA of living cells. Hoechst stains property may allow fluorescence-activated cell and chromosome sorting according to the extent of DNA synthesis which may provide a bridge between biochemical and cytologic analyses of processes related to DNA replication.
Other Notes
Contains 0.5μg/ml Hoechst bisbenzimide 33258 fluorochrome stain and thimerosal.
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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A number of applications of the detection of deoxyribonucleic acid synthesis by fluorescence microscopy are illustrated. These include (a) the analysis of sister chromatid exchanges and sister chromatid segregation at mitosis, (b) the location of chromosome regions containing deoxyribonucleic acid
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