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Merck
CN

H7285

Sigma-Aldrich

Hepatitis C virus NS4 antigen−Biotin

~1 mg/mL, ≥95% (SDS-PAGE), recombinant, expressed in E. coli

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About This Item

MDL number:
UNSPSC Code:
12352202
NACRES:
NA.32
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recombinant

expressed in E. coli

Quality Level

Assay

≥95% (SDS-PAGE)

mol wt

19 kDa (plus 114 kDa β-Gal tag)

concentration

~1 mg/mL

UniProt accession no.

storage temp.

−20°C

Gene Information

hepatitis C virus ... HCVgp1(951475)

Biochem/physiol Actions

Positive control for HCV antibodies.

Physical form

Solution in 8 M Urea, 20 mM Tris-HCl, pH 8.0, 10 mM 2-Mercaptoethanol.

Other Notes

HCV NS4a+b [1658-1863]-Galactosidase-tagged, biotin conjugate.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Miriam T Brady et al.
European journal of immunology, 33(12), 3448-3457 (2003-11-25)
The majority of hepatitis C virus (HCV) infections become chronic, despite the presence of HCV-specific cellular and humoral immune responses. We have previously suggested that IL-10-secreting antigen-specific regulatory T cells may contribute to viral persistence, and demonstrate here that peripheral
R Bartenschlager et al.
Journal of virology, 69(12), 7519-7528 (1995-12-01)
Processing of the hepatitis C virus polyprotein is mediated by host cell signalases and at least two virally encoded proteinases. Of these, the serine-type proteinase encompassing the amino-terminal one-third of NS3 is responsible for cleavage at the four sites carboxy
Margaret A Scull et al.
PLoS pathogens, 11(11), e1005297-e1005297 (2015-11-21)
The hepatitis C virus (HCV) p7 protein is required for infectious virus production via its role in assembly and ion channel activity. Although NMR structures of p7 have been reported, the location of secondary structural elements and orientation of the
Inés Romero-Brey et al.
mBio, 6(4), e00759-e00759 (2015-07-15)
Induction of membrane rearrangements in the cytoplasm of infected cells is a hallmark of positive-strand RNA viruses. These altered membranes serve as scaffolds for the assembly of viral replication factories (RFs). We have recently shown that hepatitis C virus (HCV)

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