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Merck
CN

H8898

Sigma-Aldrich

Halocarbon oil 700

Synonym(s):

Chlorotrifluoroethene homopolymer

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12162002
PubChem Substance ID:
NACRES:
NA.75
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form

liquid

Quality Level

SMILES string

FC(=C(F)Cl)F

InChI

1S/C2ClF3/c3-1(4)2(5)6

InChI key

UUAGAQFQZIEFAH-UHFFFAOYSA-N

General description

Halocarbon oil 700 is used for mounting worms on agarose pads. It facilitates movement of worms for microscopic view. The halocarbon acts as a source of oxygen and blocks desiccation during optical digital embryo sampling.

Application

Halocarbon oil 700 has been used:
  • to facilitate piercing of vitelline membranes with a glass capillary to make small holes on spider embryos
  • in microinjection procedure to track the vesicular stomatitis virus infection in Caenorhabditis elegans
  • for live imaging of embryo sample using Zeiss LSM710 confocal system

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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C. elegans: A Practical Approach, 81-81 (1999)
Lunhao Chen et al.
Frontiers in neuroscience, 12, 324-324 (2018-06-06)
Here we described an experimental protocol for in vivo imaging of macropinocytosis and subsequent intracellular events. By microinjection, we delivered fluorescence dextrans together with or without ATPγS into transparent Drosophila melanogaster embryos. Using a confocal microscope for live imaging, we
Drosophila melanogaster: Practical Uses in Cell and Molecular Biology, 514-514 (1995)
Scott Curran et al.
Developmental cell, 43(4), 480-492 (2017-11-07)
Under conditions of homeostasis, dynamic changes in the length of individual adherens junctions (AJs) provide epithelia with the fluidity required to maintain tissue integrity in the face of intrinsic and extrinsic forces. While the contribution of AJ remodeling to developmental
Multi-color FISH facilitates analysis of cell-type diversification and developmental gene regulation in the Parasteatoda spider embryo
Akiyama-Oda Y and Oda H
Development, Growth & Differentiation, 58(2), 215-224 (2016)

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