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Merck
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H9662

Sigma-Aldrich

Anti-hBRM/hSNF2α (LG-14) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41
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biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 230 kDa

species reactivity

human

technique(s)

western blot: 1:1,000 using extracts of 293T cells transfected with hBRM

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SMARCA2(6595)

Immunogen

synthetic peptide corresponding to amino acids 1537-1550 of human BRM conjugated to KLH through an N-terminal added cysteine.

Application

Anti-hBRM/hSNF2a (KR-17) antibody produced in rabbit is suitable for:
  • indirect immunofluorescence: 1μg/mL using paraformaldehyde/Triton fixed hBrm-transfected 293T cell line
  • microarray
  • western blot at a concentration of 0.15μg/mL using nuclear extracts of the 293T cell line

Biochem/physiol Actions

Anti-hBrm/hSNF2α (LG-14) recognizes hBrm (230 kDa).
Chromatin remodeling complexes from various organisms, structurally contain related catalytic subunits, but differ in the way in which they manipulate chromatin. Three families of complexes have been described the SWI/SNF family, ISWI family, and Mi-2 family. The SWI/SNF family of ATP-dependent remodeling complexes was identified in yeast, drosophila, and human. It causes nucleosomes to change structure and/or position in order to allow transcriptional activators to gain access to their target sites. In humans, two conserved ATPase subunits have been identified as hBrm (also designated hSNF2α) and Brg1 (also designated as SNF2β). Components of the hSWI/SNF complexes have been implicated in a range of cellular events including gene activation, regulation of cell growth, and development. Brg1 and hBrm enhances transcriptional activation by glucocorticoid receptors. The remodeling complexes were traditionally associated with transcriptional activation. However, SWI/SNF has been found associated with repressor complexes, such as HDAC (histone deacetylase) and Rb (retinoblastoma) in a complex that leads to cell cycle arrest, suggesting that they are associated with transcriptional repression.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at −20 °C. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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S R Biggar et al.
The EMBO journal, 18(8), 2254-2264 (1999-04-16)
Chromatin presents a significant obstacle to transcription, but two means of overcoming its repressive effects, histone acetylation and the activities of the Swi-Snf complex, have been proposed. Histone acetylation and Swi-Snf activity have been shown to be crucial for transcriptional
P Sudarsanam et al.
Trends in genetics : TIG, 16(8), 345-351 (2000-07-25)
The Swi/Snf family of nucleosome-remodeling complexes has been shown to play important roles in gene expression throughout eukaryotes. Genetic and biochemical studies previously suggested that Swi/Snf activates transcription by remodeling nucleosomes, thereby permitting increased access of transcription factors for their
W Wang et al.
The EMBO journal, 15(19), 5370-5382 (1996-10-01)
We have purified distinct complexes of nine to 12 proteins [referred to as BRG1-associated factors (BAFs)] from several mammalian cell lines using an antibody to the SWI2-SNF2 homolog BRG1. Microsequencing revealed that the 47 kDa BAF is identical to INI1.
L A Boyer et al.
The Journal of biological chemistry, 275(25), 18864-18870 (2000-04-26)
ATP-dependent chromatin remodeling enzymes antagonize the inhibitory effects of chromatin. We compare six different remodeling complexes: ySWI/SNF, yRSC, hSWI/SNF, xMi-2, dCHRAC, and dNURF. We find that each complex uses similar amounts of ATP to remodel nucleosomal arrays at nearly identical
C Muchardt et al.
The EMBO journal, 15(13), 3394-3402 (1996-07-01)
In yeast, the SNF/SWI complex is believed to regulate transcription by locally altering the chromatin structure. At the present time, three human homologues of yeast SNF/SWI proteins have been characterized: hbrm and BRG-1, homologues of SNF2/SWI2, and hSNF5, a homologue

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